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Series GSE225622 Query DataSets for GSE225622
Status Public on Jun 07, 2023
Title Effect of androgen; gonad- and androgen receptor on gene expression in mouse kidney and liver
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate mechanisms underlying renal sex differences, we performed whole kidney RNA-seq on wild type C57BL/6J mice (Jackson Laboratory) at postnatal 0, 2, 4, 8 and 79 weeks old. We also profiled wild type adult C57BL/6NCrl (Charles River Laboratory) and adult castrated, ovariectomized mice when the surgeries for gonadal removal were performed at the age of around 3 weeks old. Tissue specific Ar (androgen receptor), Tbx10 and Neurog2 knockout mice were maintained at F1 cross Arc/c floxed allele to Six2TGC/+ CRE strain.
To examine the acute response of testosterone, we injected testosterone to adult castrated and ovariectomized mice and collected kidneys 24 hours later for bulk RNA-seq. To compare the mechanisms of Ar in regulating sex differences systemetically or locally in kidney and liver, we obtained bulk RNA-seq for kidneys and livers from ubiquitous, nephron-specific and hepatocyte-specific knockout of Ar by Sox2CRE/+, Six2TGC/+ and Albumin-CRE mouse strain respectively.
 
Overall design Total RNA were collected for RNA-seq from minced 1) kidneys of C57BL/6J (Jackson Laboratory) at postnatal age 0, 2, 4, 8 and 79 weeks old; 2) kidneys of wild type C57BL/6NCrl mice (Charles River Laboratory), and those underwent castration or ovariectomy at around 3 weeks old that were injected with testosterone or oil control at 8~11 weeks old; 3) kidneys of adult conditional knockout by nephron-specific CRE strain Six2TGC/+ for Ar, Tbx10 and Neurog2 ; 4) kidneys and livers from ubiquitous removal of Ar by Sox2-CRE strain; 5) livers of hepatocyte-specific removal of Ar by Albumin CRE for both sexes.
 
Contributor(s) Liu J, McMahon A
Citation(s) 37205355
Submission date Feb 20, 2023
Last update date Jun 07, 2023
Contact name Jing Liu
E-mail(s) jing.liu@med.usc.edu
Phone 3034428077
Organization name University of Southern California
Department Department of Regenerative Medicine & Stem Cell Biology
Lab Andy McMahon lab
Street address 1425 San Pablo St.
City Los Angeles
State/province California
ZIP/Postal code 90033
Country USA
 
Platforms (3)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL28330 DNBSEQ-T7 (Mus musculus)
Samples (182)
GSM7051385 31_F1_P0_C57BL/6J
GSM7051386 32_F2_P0_C57BL/6J
GSM7051387 73_fp0_3_C57BL/6J
Relations
BioProject PRJNA936831

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Supplementary file Size Download File type/resource
GSE225622_146_155_all.gene_counts.txt.gz 1.4 Mb (ftp)(http) TXT
GSE225622_156_188_all.gene_counts.txt.gz 2.3 Mb (ftp)(http) TXT
GSE225622_1_WashU_50SE_counts.txt.gz 6.4 Mb (ftp)(http) TXT
GSE225622_2_BGI_PE150_counts.txt.gz 4.8 Mb (ftp)(http) TXT
GSE225622_3_WashU_PE150_counts.txt.gz 5.7 Mb (ftp)(http) TXT
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