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| Status |
Public on Dec 01, 2023 |
| Title |
Integrating into dairy goats chromatin accessible sites during lactation promotes transgene expression efficiency |
| Organism |
Capra hircus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Combining RNA-seq with ATAC-seq, we screened the open chromatin of dairy goat mammary epithelial cells during lactation and then integrated human lysozyme into various open regions using the CRISPR/Cas9 system. A comparison of lysozyme gene expression and integration efficiency showed that chromatin accessibility at target sites was significantly positively correlated with gene insertion efficiency, and the insertion of foreign genes into highly accessible chromatin regions could enhance its expression.
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| Overall design |
3 dry period and 3 lactation period samples for Capra hircus species were sequenced using ATAC-Seq and RNA-seq by the Beijing Genomics institution (Nanjing, China).
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| Contributor(s) |
Gao Y, Zhu Z, Han J |
| Citation missing |
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| Submission date |
Feb 20, 2023 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Zhenliang Zhu |
| E-mail(s) |
zhuzhenliang@nwafu.edu.cn
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| Phone |
+86 18829351022
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| Organization name |
Northwest A&F University
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| Street address |
3 Taicheng Road
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| City |
Yangling |
| ZIP/Postal code |
712100 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA936887 |
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