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Status |
Public on Aug 07, 2023 |
Title |
CD8+ cells and small viral reservoirs facilitate post-ART SIV control in MHC-M3+ Mauritian cynomolgus macaques |
Organism |
Macaca fascicularis |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Sustainable HIV remission after antiretroviral therapy (ART) withdrawal, or post-treatment control (PTC), remains a top priority in the HIV field. We observed surprising PTC in an MHC-haplomatched cohort of SIVmac239+ Mauritian cynomolgus macaques (MCMs) initiated on ART at two weeks post-infection (wpi). For six months after ART withdrawal, we observed undetectable or transient viremia in seven of eight MCMs. In vivo CD8α depletion induced rebound in all animals, indicating the PTC was mediated by CD8α+ cells. We found that MCMs had a smaller acute viral reservoir than a cohort of identically infected rhesus macaques, a population that rarely develops PTC. The mechanisms by which unusually small viral reservoirs and CD8α+ cell-mediated virus suppression enable PTC can be investigated using this model of PTC in MHC-haplomatched MCMs. If the immunological mechanisms that can engender PTC are defined, this model could designate therapeutic targets for HIV remission strategies in humans.
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Overall design |
Comparative gene expression proifiling analysis of RNA-seq data for CD8+ T cells isolated from peripheral blood mononuclear cells (PBMCs) of SIV+ Mauritian Cynomolgus macaques.
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Contributor(s) |
Harwood OE, Matschke LM, Moriarty RV, Balgeman AJ, Weaver AJ, Ellis-Connell AL, Weiler AM, Winchester LC, Fletcher CV, Friedrich TC, Keele BF, O’Connor DH, Lang JD, Reynolds MR, O’Connor SL |
Citation(s) |
36909458, 37747933 |
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Submission date |
Feb 21, 2023 |
Last update date |
Oct 18, 2023 |
Contact name |
Shelby O'Connor |
E-mail(s) |
slfeinberg@wisc.edu
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Organization name |
University of Wisconsin-Madison
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Street address |
555 Science Drive
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City |
Madison |
State/province |
WI |
ZIP/Postal code |
53711 |
Country |
USA |
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Platforms (1) |
GPL28212 |
Illumina NovaSeq 6000 (Macaca fascicularis) |
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Samples (16)
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Relations |
BioProject |
PRJNA937379 |
Supplementary file |
Size |
Download |
File type/resource |
GSE225770_RAW.tar |
34.1 Mb |
(http)(custom) |
TAR (of TXT) |
GSE225770_salmon.merged.gene_counts.xlsx |
1.8 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
Processed data are available on Series record |
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