|
| Status |
Public on Mar 27, 2023 |
| Title |
Effect of frataxin silencing by RNAi on 4, 7 and 14 days old worms |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
The study aimed to investigate the mechanisms that promote compensatory pro-longevity responses during mild mitochondrial stress in Caenorhabditis elegans. This was achieved by performing a DNA microarray analysis to compare gene expression profiles of wild-type and mild mitochondrial-stressed long-lived worms at days 4, 7, and 14 after birth, in five replicates. The worms were fed with either an empty-vector control (pL4440) or vector-expressing dsRNA against nematode frataxin (frh-1) to induce mild mitochondrial stress, for 3 consecutive generation starting from eggs.
|
| |
|
| Overall design |
frh-1 silenced and control group were collected at days 4, 7, and 14 after birth in 5 independent replicates.
|
| Web link |
https://doi.org/10.1016/j.isci.2023.106448
|
| |
|
| Contributor(s) |
Schiavi A, Ventura N |
| Citation(s) |
37020951 |
| Submission date |
Feb 22, 2023 |
| Last update date |
Apr 17, 2023 |
| Contact name |
Natascia Ventura |
| Organization name |
Heinrich Heine University and the IUF- Leibniz Research Institute for Environmental Medicine
|
| Street address |
Auf'm Hennekamp 50
|
| City |
Düsseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL19230 |
[EleGene-1_0-st] Affymetrix C. elegans Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (30)
|
|
| Relations |
| BioProject |
PRJNA937591 |
Less...
More...