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| Status |
Public on Nov 20, 2023 |
| Title |
4C-seq analysis in TAL1-FKBP12 Jurkat cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
4C analysis was performed in TAL1-FKBP12 Jurkat cells to analyze chromatin-chromatin interactions after DMSO or dTAG-13 treatment.
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| Overall design |
Restriction enzyme digestion was performed with N1aIII (NEB) followed by overnight ligation and subsequent reverse crosslinking. DNA fragments were purified to yield the 3C template. 3C template was then subjected to a second restriction enzyme digestion by DpnII (NEB), overnight ligation and sample purification were repeated to yield the 4C template. Library preparation involved two PCR steps to amplify the fragments ligated to the sequence of interest followed by a second PCR to incorporate sequencing adapters.
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| |
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| Contributor(s) |
Ong JZ, Tan TK, Sanda T |
| Citation(s) |
37855064 |
| |
| Submission date |
Feb 23, 2023 |
| Last update date |
Nov 21, 2023 |
| Contact name |
Tze King Tan |
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute
|
| Lab |
Takaomi Sanda Lab
|
| Street address |
14 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE225941 |
Regulatory mechanisms and context-dependent roles of TAL1 in TALL |
|
| Relations |
| BioProject |
PRJNA938075 |
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