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Series GSE225942 Query DataSets for GSE225942
Status Public on May 24, 2023
Title Neuro-PASC is characterized by enhanced CD4+ and diminished CD8+ T cell responses to SARS-CoV-2 Nucleocapsid protein
Organism Homo sapiens
Experiment type Other
Summary Many people experiencing long COVID symptoms, or post-acute sequelae of SARS-CoV-2 infection (PASC), suffer from debilitating neurologic symptoms (Neuro-PASC). Although symptoms of Neuro-PASC are widely documented, it is still unclear whether virus-specific adaptive immunity is affected in these individuals. We report that Neuro-PASC patients exhibit distinct immunological signatures composed of elevated CD4+ T cell responses toward the C-terminal region of SARS-CoV-2 Nucleocapsid protein when examined both functionally and using TCR sequencing. In contrast, Neuro-PASC patients also have diminished activation of CD8+ memory T cells and increased IL-6 production to Nucleocapsid. Elevated plasma immunoregulatory and reduced pro-inflammatory and antiviral response signatures were evident in Neuro-PASC patients compared with COVID convalescent controls without lasting symptoms, correlating with worse neurocognitive dysfunction. These data provide new insight into the impact of virus-specific cellular immunity on the pathogenesis of long COVID and pave the way for the rational design of predictive biomarkers and therapeutic interventions.
 
Overall design Genomic DNA samples from Neuro-PASC (NP; experimental) and COVID convalescent controls (CC; control) underwent TCR-sequencing through the Adaptive Biotechnologies ImmunoSEQ platform.
***Adaptive Biotechnologies does not release raw data.***
Web link https://www.frontiersin.org/articles/10.3389/fimmu.2023.1155770/full
 
Contributor(s) Visvabharathy L, Koralnik I
Citation(s) 37313412
Submission date Feb 23, 2023
Last update date Aug 23, 2023
Contact name Igor Koralnik
E-mail(s) lavanya.visvabharathy@northwestern.edu
Organization name Northwestern University
Department Neurology
Street address 320 E. Superior St, Searle Rm. 7-450
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platforms (1)
GPL15520 Illumina MiSeq (Homo sapiens)
Samples (38)
GSM7060726 PBMC, COVID convalescent CC47
GSM7060727 PBMC, COVID convalescent CC35
GSM7060728 PBMC, COVID convalescent CC34
Relations
BioProject PRJNA938055

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Supplementary file Size Download File type/resource
GSE225942_RAW.tar 356.2 Mb (http)(custom) TAR (of TSV)
Raw data not provided for this record
Processed data provided as supplementary file

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