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| Status |
Public on Nov 20, 2023 |
| Title |
ChIP-seq analysis for TAL1-FKBP12 Jurkat cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ChIP-seq analysis was performed in a Jurkat cell line to analyze DNA bindings of TAL1-FKBP12 protein using an anti-HA antibody after DMSO or dTAG-13 treatment.
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| Overall design |
The cells were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP), subjected for library construction, and sequenced by the DNBSEQ platform. A sample of whole cell extract (input) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against HA (Cat#3724, Cell Signalling Technology).
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| Contributor(s) |
Ong JZ, Tan TK, Sanda T |
| Citation(s) |
37855064 |
| |
| Submission date |
Feb 27, 2023 |
| Last update date |
Nov 21, 2023 |
| Contact name |
Tze King Tan |
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute
|
| Lab |
Takaomi Sanda Lab
|
| Street address |
14 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE225941 |
Regulatory mechanisms and context-dependent roles of TAL1 in TALL |
|
| Relations |
| BioProject |
PRJNA939156 |
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