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Series GSE226356 Query DataSets for GSE226356
Status Public on Mar 03, 2023
Title YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells [RPPA]
Organisms Homo sapiens; Mus musculus
Experiment type Protein profiling by protein array
Summary In heterogeneous head and neck cancer (HNC), subtype-specific treatment regimens are currently missing. An integrated analysis of patient HNC subtypes using single-cell sequencing and proteome profiles revealed an epithelial-mesenchymal transition (EMT) signature within the epithelial cancer-cell population. The EMT signature coincided with PI3K/mTOR inactivation in the mesenchymal subtype. Conversely, the signature was suppressed in epithelial cells of the basal subtype which exhibits hyperactive PI3K/mTOR signalling. We further identified YBX1 phosphorylation, downstream of the PI3K/mTOR pathway, restrains basal-like cancer cell proliferation. In contrast, YBX1 acts as a safeguard against the proliferation-to-invasion switch in mesenchymal-like epithelial cancer cells, and its loss accentuates partial-EMT and in vivo invasion. Interestingly, phospho-YBX1 that is mutually exclusive to partial-EMT, emerged as a prognostic marker for overall patient outcomes. Expression profiling by reverse-phase protein array were performed to explore the underlying molecular mechanisms.
Overall design The reverse-phase protein array analysis of head and neck cancer (HNC) cell lines SCC15 and SCC25 upon YBX1 knockout. The reverse-phase protein array analysis of normal oral cells (OKF6) and HNC cells (SCC15 and SCC25). The reverse-phase protein array analysis of mouse tissues (wildtype, Grhl3cKO, Pik3ca, Pik3caGrhl3cKO tumours and adjacent tissues)
Web link
Contributor(s) Darido C, Bai Y
Citation(s) 36949044
Submission date Mar 01, 2023
Last update date Jun 02, 2023
Contact name Charbel Darido
Organization name Peter MacCallum Cancer Center
Department Cancer Research
Lab Head and Neck Cancer Signaling Lab
Street address 305 Grattan street
City Melbourne
State/province VIC
ZIP/Postal code 3000
Country Australia
Platforms (1)
GPL33200 Reverse Phase Protein Array (RPPA) - The University of Edinburgh
Samples (32)
GSM7073847 OKF6, rep 1
GSM7073848 OKF6, rep 2
GSM7073849 SCC15, YBX1KO, DOXneg, EGFneg, rep 1
This SubSeries is part of SuperSeries:
GSE226357 YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells.
BioProject PRJNA940055

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE226356_PMR-125_NormToSec.RawData.xlsx 58.2 Kb (ftp)(http) XLSX
Processed data included within Sample table

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