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GEO help: Mouse over screen elements for information. |
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Status |
Public on Mar 03, 2023 |
Title |
YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells [RPPA] |
Organisms |
Homo sapiens; Mus musculus |
Experiment type |
Protein profiling by protein array
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Summary |
In heterogeneous head and neck cancer (HNC), subtype-specific treatment regimens are currently missing. An integrated analysis of patient HNC subtypes using single-cell sequencing and proteome profiles revealed an epithelial-mesenchymal transition (EMT) signature within the epithelial cancer-cell population. The EMT signature coincided with PI3K/mTOR inactivation in the mesenchymal subtype. Conversely, the signature was suppressed in epithelial cells of the basal subtype which exhibits hyperactive PI3K/mTOR signalling. We further identified YBX1 phosphorylation, downstream of the PI3K/mTOR pathway, restrains basal-like cancer cell proliferation. In contrast, YBX1 acts as a safeguard against the proliferation-to-invasion switch in mesenchymal-like epithelial cancer cells, and its loss accentuates partial-EMT and in vivo invasion. Interestingly, phospho-YBX1 that is mutually exclusive to partial-EMT, emerged as a prognostic marker for overall patient outcomes. Expression profiling by reverse-phase protein array were performed to explore the underlying molecular mechanisms.
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Overall design |
The reverse-phase protein array analysis of head and neck cancer (HNC) cell lines SCC15 and SCC25 upon YBX1 knockout. The reverse-phase protein array analysis of normal oral cells (OKF6) and HNC cells (SCC15 and SCC25). The reverse-phase protein array analysis of mouse tissues (wildtype, Grhl3cKO, Pik3ca, Pik3caGrhl3cKO tumours and adjacent tissues)
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Web link |
https://www.nature.com/articles/s41467-023-37161-0
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Contributor(s) |
Darido C, Bai Y |
Citation(s) |
36949044 |
Submission date |
Mar 01, 2023 |
Last update date |
Jun 02, 2023 |
Contact name |
Charbel Darido |
E-mail(s) |
charbel.darido@petermac.org
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Organization name |
Peter MacCallum Cancer Center
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Department |
Cancer Research
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Lab |
Head and Neck Cancer Signaling Lab
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Street address |
305 Grattan street
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City |
Melbourne |
State/province |
VIC |
ZIP/Postal code |
3000 |
Country |
Australia |
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Platforms (1) |
GPL33200 |
Reverse Phase Protein Array (RPPA) - The University of Edinburgh |
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Samples (32)
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GSM7073850 |
SCC15, YBX1KO, DOXneg, EGFneg, rep 2 |
GSM7073851 |
SCC15, YBX1KO, DOXneg, EGFpos, rep 1 |
GSM7073852 |
SCC15, YBX1KO, DOXneg, EGFpos, rep 2 |
GSM7073853 |
SCC15, YBX1KO, DOXpos, EGFneg, rep 1 |
GSM7073854 |
SCC15, YBX1KO, DOXpos, EGFneg, rep 2 |
GSM7073855 |
SCC15, YBX1KO, DOXpos, EGFpos, rep 1 |
GSM7073856 |
SCC15, YBX1KO, DOXpos, EGFpos, rep 2 |
GSM7073857 |
SCC25, YBX1KO, DOXneg, EGFneg, rep 1 |
GSM7073858 |
SCC25, YBX1KO, DOXneg, EGFneg, rep 2 |
GSM7073859 |
SCC25, YBX1KO, DOXneg, EGFpos, rep 1 |
GSM7073860 |
SCC25, YBX1KO, DOXneg, EGFpos, rep 2 |
GSM7073861 |
SCC25, YBX1KO, DOXpos, EGFneg, rep 1 |
GSM7073862 |
SCC25, YBX1KO, DOXpos, EGFneg, rep 2 |
GSM7073863 |
SCC25, YBX1KO, DOXpos, EGFpos, rep 1 |
GSM7073864 |
SCC25, YBX1KO, DOXpos, EGFpos, rep 2 |
GSM7073865 |
Wildtype C57BL/6 mouse, rep 1 |
GSM7073866 |
Wildtype C57BL/6 mouse, rep 2 |
GSM7073867 |
Grhl3cKO C57BL/6 mouse, rep 1 |
GSM7073868 |
Grhl3cKO C57BL/6 mouse, rep 2 |
GSM7073869 |
Grhl3cKO C57BL/6 mouse, rep 3 |
GSM7073870 |
Pik3caKI C57BL/6 mouse, rep 1 |
GSM7073871 |
Pik3caKI C57BL/6 mouse, rep 2 |
GSM7073872 |
Pik3caKI C57BL/6 mouse, rep 3 |
GSM7073873 |
Pik3caKI, Grhl3cKO C57BL/6 mouse, tumour, rep 1 |
GSM7073874 |
Pik3caKI, Grhl3cKO C57BL/6 mouse, adjacent, rep 1 |
GSM7073875 |
Pik3caKI, Grhl3cKO C57BL/6 mouse, tumour, rep 2 |
GSM7073876 |
Pik3caKI, Grhl3cKO C57BL/6 mouse, adjacent, rep 2 |
GSM7073877 |
Pik3caKI, Grhl3cKO C57BL/6 mouse, tumour, rep 3 |
GSM7073878 |
Pik3caKI, Grhl3cKO C57BL/6 mouse, adjacent, rep 3 |
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This SubSeries is part of SuperSeries: |
GSE226357 |
YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells. |
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Relations |
BioProject |
PRJNA940055 |
Supplementary file |
Size |
Download |
File type/resource |
GSE226356_PMR-125_NormToSec.RawData.xlsx |
58.2 Kb |
(ftp)(http) |
XLSX |
Processed data included within Sample table |
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