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| Status |
Public on Mar 13, 2023 |
| Title |
Osr1 controls the pro-regenerative response of Fibro-Adipogenic Progenitors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To study the requirement of Osr1 expression for FAP function during skeletal muscle regeneration.
Methods: We sequenced total mRNAs isolated from adult FAPs FACS sorted from 3 and 7 day post injured hindlimb skeletal muscle of Ctrl (Osr1flox/+ CAGG Cre+) and Osr1 cKO (Osr1flox/flox CAGG Cre+) mice.
Results: Loss of Osr1 leads to pro-fibrotic orientation of FAPs and impairs both FAP-macrophage and FAP-MuSCs communication network resulting in impaired regenerative myogenesis and persistent fibrosis.
Conclusions: Osr1 is a key transcriptional regulator of FAP regenerative function protecting FAPs from assuming a detrimental pro-fibrotic and anti-myogenic state.
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| Overall design |
Sequencing was performed on RNAs isolated from adult FAPs FACS sorted from 3 and 7 days post injured hindlimb skeletal muscle of Ctrl (Osr1flox/+ CAGG Cre+) and Osr1 cKO (Osr1flox/flox CAGG Cre+) mice.
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| Web link |
https://www.nature.com/articles/s41536-023-00291-6
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| Contributor(s) |
Kotsaris G, Stricker S |
| Citation(s) |
37019910 |
| BioProject |
PRJNA938360 |
| |
| Submission date |
Mar 06, 2023 |
| Last update date |
Apr 27, 2023 |
| Contact name |
Georgios Kotsaris |
| E-mail(s) |
georgios.kotsaris@fu-berlin.de
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| Organization name |
Freie Universität Berlin
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| Street address |
Thielallee, 63
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| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (8)
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