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| Status |
Public on Aug 11, 2023 |
| Title |
Chromatin mechanism of DELLA-mediated gene repression and Gibberellin-induced gene activation in rice |
| Organism |
Oryza sativa Japonica Group |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
GA treatment of wild-type rice and use of DELLA protein mutant for RNA-seq and ChIP-seq. Explaining the chromatin mechanisms of DELLA-mediated gene repression and GA signalling-induced gene activation.
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| Overall design |
To investigate the chromatin mechanism of DELLA-mediated gene repression. We performed H3K27me3 ChIP-seq analysis and transcriptome analysis using 12-day-old mutant seedlings of DELLA and the corresponding wild-type seedlings. To study the GA-induced genome-wide histone modification changes, we performed H3K27me3 ChIP-seq analysis of rice plants treated with 100uM GA for 6h and then we analyzed the transcriptomes of rice seedlings treated with or without 100uM GA for1h.
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| Web link |
http://10.15252/embj.2023114220
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| Contributor(s) |
Li J, Li Q, Zhou D |
| Citation(s) |
37691541 |
| |
| Submission date |
Mar 07, 2023 |
| Last update date |
Nov 12, 2023 |
| Contact name |
Qi Li |
| E-mail(s) |
liqi0427@webmail.hzau.edu.cn
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| Organization name |
Huazhong Agricultural University
|
| Department |
Hongshan District
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| Street address |
Lion Rock Street
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| City |
Wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (2) |
| GPL13834 |
Illumina HiSeq 2000 (Oryza sativa Japonica Group) |
| GPL27860 |
Illumina NovaSeq 6000 (Oryza sativa Japonica Group) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA941831 |
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