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Series GSE22725 Query DataSets for GSE22725
Status Public on Jul 07, 2010
Title Strome AB8898_H3K9ME3339901_N2_EEMB
Project modENCODE
Organism Caenorhabditis elegans
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary modENCODE_submission_2339
This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents.
We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf

Keywords: CHIP-chip
 
Overall design EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: Early Embryo; Genotype: wild type; Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 1; EXPERIMENTAL FACTORS: Developmental Stage Early Embryo; Developmental Stage Early Embryo; Strain N2; Antibody AB8898 H3K9ME3:339901 (target is H3K9me3); Strain N2; temperature 20
Web link http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
http://www.modencode.org
 
Contributor(s) Egelhofer T, Strome S
Citation missing Has this study been published? Please login to update or notify GEO.
BioProject PRJNA63455
Submission date Jul 05, 2010
Last update date Feb 02, 2015
Contact name DCC modENCODE
E-mail(s) help@modencode.org
Phone 416-673-8579
Organization name Ontario Institute for Cancer Research
Lab modENCODE DCC
Street address MaRS Centre, South Tower, 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
 
Platforms (1)
GPL8647 080922_modEncode_Caenorhabditis elegans_2M tiling chip_HX1
Samples (1)
GSM562725 AB8898_H3K9ME3339901_N2_EEMB_1 extraction1_array1

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22725_RAW.tar 188.7 Mb (http)(custom) TAR (of PAIR, WIG)
Processed data provided as supplementary file

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