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Status |
Public on Mar 19, 2023 |
Title |
Genome-wide mapping of NRF1 (NFE2L1) binding in HCT116 cells upon proteasome inhibition. |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The maintenance of protein homeostasis is an essential characteristic of life. Transcription factor NRF1 (NFE2L1) has been reported to be activated by proteasome dysfunction, although the genome-wide target genes are poorly understood. Using ChIP-seq analysis, we found a potential association between NRF1 and autophagy. Our findings highlight the new activation mechanism of autophagy through gene regulation under proteasome dysfunction.
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Overall design |
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for transcription factor NRF1 in HCT116 cells treated with 1 µM MG132 for 16 h.
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Contributor(s) |
Hatanaka A, Watanabe A, Waku T, Kobayashi A |
Citation(s) |
37658135 |
Submission date |
Mar 14, 2023 |
Last update date |
Sep 15, 2023 |
Contact name |
Akira Kobayashi |
E-mail(s) |
akobayas@mail.doshisha.ac.jp
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Phone |
+81-774-65-6273
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Organization name |
Doshisha University
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Street address |
1-3 Tatara Miyakodani
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City |
Kyotanabe |
ZIP/Postal code |
610-0394 |
Country |
Japan |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (2) |
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Relations |
BioProject |
PRJNA944699 |