 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 18, 2023 |
| Title |
Transcriptomics analysis of a zebrafish model of Combined Saposin Deficiency |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Combined Saposin Deficiency is a lysosomal storage disease with multiple-organ pathologies that often involve neurodegeneration and early mortality. To investigate the mechanisms driving saposin loss-associated demyelination, the brains of psap-/- zebrafish and wildtype siblings were analyzed via RNA-seq.
|
| |
|
| Overall design |
A zebrafish model of Combined Saposin Deficiency was generated using CRISPR-Cas9 targeting the prosaposin (psap) gene, which codes for saposins A-D. Mutagenized alleles were identified by DNA fragment analysis and Sanger sequencing, and fish carrying the mutations of interest were propagated to homozygosity. RNA was isolated from the brains of four months-post-fertilization psap-/- zebrafish and wildtype siblings.
|
| Web link |
https://pubmed.ncbi.nlm.nih.gov/37183607/
|
| |
|
| Contributor(s) |
Zhang T, Stubben C, Peterson RT |
| Citation(s) |
37183607 |
| Submission date |
Mar 16, 2023 |
| Last update date |
Jun 20, 2023 |
| Contact name |
Tejia Zhang |
| E-mail(s) |
tejia.zhang@pharm.utah.edu
|
| Organization name |
University of Utah
|
| Department |
Pharmacology and Toxicology
|
| Lab |
Peterson
|
| Street address |
30 South 2000 East
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
|
| Samples (8)
|
| GSM7102578 |
Brain, wildtype, 4 months-post-fertilization, replicate 1 [15628X1] |
| GSM7102579 |
Brain, psap knockout, 4 months-post-fertilization, replicate 1 [15628X2] |
| GSM7102580 |
Brain, wildtype, 4 months-post-fertilization, replicate 2 [15628X3] |
| GSM7102581 |
Brain, psap knockout, 4 months-post-fertilization, replicate 2 [15628X4] |
| GSM7102582 |
Brain, wildtype, 4 months-post-fertilization, replicate 3 [15628X5] |
| GSM7102583 |
Brain, psap knockout, 4 months-post-fertilization, replicate 3 [15628X6] |
| GSM7102584 |
Brain, wildtype, 4 months-post-fertilization, replicate 4 [15628X7] |
| GSM7102585 |
Brain, psap knockout, 4 months-post-fertilization, replicate 4 [15628X8] |
|
| Relations |
| BioProject |
PRJNA945489 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE227551_WT_psap_DESeq.xlsx |
11.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...