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Series GSE227758 Query DataSets for GSE227758
Status Public on Sep 06, 2023
Title Transcriptomic profile of dermal and hypodermal CCR2+ macrophages, CCR2- macrophages and dendritic cells from C57BL/6 mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In order to explore and compare the transcriptomic profile of CCR2+ macrophages, CCR2- macrophages and dendritic cells present in the dermis and hypodermis of C57BL/6 mice, isolated cells were subjected to RNA-sequencing analysis.
 
Overall design Back skins from C57BL/6 female mice were harvested and the dermal and hypodermal layers isolated by mechanical force and enzymatic treatments. Cells were liberated from each skin layers after enzymatic digestion of the tissues. Macrophages (CD45+ CD11b+ EpCAM–Ly6C-lo CD11c-lo/mid CD64+) expressing or not CCR2 as well as dendritic cells (CD45+ CD11b+ EpCAM–Ly6C-lo CD11c-lo/mid CD64- MHC-II+) were isolated by fluorescence-activated cell sorting (FACS) and their transcriptome assessed by RNA-sequencing.
 
Contributor(s) Voisin B, Doebel T, Hu Y, Yan C, Nagao K
Citation(s) 37402364
Submission date Mar 20, 2023
Last update date Sep 07, 2023
Contact name Keisuke Nagao
Organization name National Institutes of Health
Street address 10 Center Dr, Bldg 10, 12N240B
City Bethesda
State/province MD
ZIP/Postal code 20852
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (18)
GSM7107638 CCR2+ macrophages, dermis, replicate 1
GSM7107639 CCR2+ macrophages, dermis, replicate 2
GSM7107640 CCR2+ macrophages, dermis, replicate 3
This SubSeries is part of SuperSeries:
GSE228445 Dermal and hypodermal macrophages and dendritic cells
Relations
BioProject PRJNA946769

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE227758_RAW.tar 22.9 Mb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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