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| Status |
Public on Apr 03, 2024 |
| Title |
Single-cell RNA-seq data of human PBMC from Myasthenia Gravis patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Myasthenia Gravis (MG) is a chronic autoimmune disorder characterized by severe muscle weakness. However, the seronegative MG patient subgroup, which accounts for about 15% of MG patients, remains challenging diagnosis, and the immunopathology is understudied. Therefore, we performed single-cell RNA-sequencing analysis by peripheral blood and plasma proteome analysis for the seronegative MG patients. Numerical abnormalities were observed in multiple immune cells including B-cells compared to healthy controls (HC) and correlated with disease activities. Additionally, plasma protein of CD22 that was reported to be expressed in the lineage of B-cell maturation, such as mature B-cells and memory B-cells was decreased compared to HC, and had correlation with disease severity, B-cells frequency, and the RNA expression level. These results suggest that the seronegative MG patients have immune disorders centered on B-cells. This is the first large-scale and innovative study to elucidate the immunopathology of the seronegative MG patients.
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| Overall design |
Examination of gene expression profile of human PBMC from Myasthenia Gravis patients.
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| Contributor(s) |
Suzuki S, Ito T, Maejima A, Okuzono Y |
| Citation(s) |
38711503 |
| |
| Submission date |
Mar 21, 2023 |
| Last update date |
May 15, 2024 |
| Contact name |
Yuumi Okuzono |
| E-mail(s) |
yuumi.okuzono@takeda.com
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| Phone |
810466322618
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| Organization name |
Takeda Pharmaceutical Company Limited
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| Street address |
26-Muraoka-higashi 2-chome
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| City |
FUJISAWA |
| State/province |
KANAGAWA |
| ZIP/Postal code |
251-8555 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA964702 |
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