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| Status |
Public on Mar 27, 2023 |
| Title |
Olfactory and neuropeptide inputs to appetite neurons in the arcuate nucleus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report RNA sequencing of single Agrp neurons isolated from the arcuate nucleus of the hypothalamus. Analyses of Agrp neuron transcriptomes reveals differential expression of receptors for multiple neuromodulators. Neuroanatomical mapping of known ligands of the receptors define subsets of neurons directly upstream of AgRP neurons in specific brain areas.
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| |
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| Overall design |
Isolation of fluorescently labelled individuals cells disociated from the mouse arcuate nuclues. Cells were visualized and isolated using a micropipette under a microscope, followed by cell lysis, RT, and cDNA synthesis.
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| Contributor(s) |
Buck L |
| Citation(s) |
36909633 |
| Submission date |
Mar 21, 2023 |
| Last update date |
Jul 10, 2023 |
| Contact name |
Linda Buck |
| E-mail(s) |
lbuck@fhcrc.org
|
| Phone |
2066676316
|
| Organization name |
Fred Hutchinson Cancer Research Centre
|
| Department |
Basic Sciences
|
| Lab |
Buck Lab
|
| Street address |
1100 fairview ave North
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA947338 |
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