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| Status |
Public on Jun 13, 2023 |
| Title |
Effect of BAF disruption on chromatin accessibility in activated CD8+ T cells [ATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To investigate the role of cBAF in regulation of Tbet binding, polyclonal naive CD8 T cells were stimulated for 48h with anti-CD3, CD28, and hIL-2.
Cells were then treated with DMSO, ACBI1, or BRM014 for 6 hours, and treated with recombinant mouse IL-12 for the last two hours of inhibitor treatment. Chromatin accessibility profiling was performed via ATAC-seq.
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| |
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| Overall design |
ATAC-seq on WT CD8+ T cells activated with anti-CD3, anti-CD28, and hIL-2 for 48h in vitro.
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| Contributor(s) |
McDonald B, Chick B |
| Citation(s) |
37315534 |
| |
| Submission date |
Mar 24, 2023 |
| Last update date |
Sep 12, 2023 |
| Contact name |
Bryan McDonald |
| E-mail(s) |
bmcdonald@salk.edu
|
| Organization name |
Salk Institute for Biological Studies
|
| Street address |
10010 N Torrey Pines Rd
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| GSM7116304 |
WT cells, 48h activated in vitro; Untreated [Control_Rep1] |
| GSM7116305 |
WT cells, 48h activated in vitro; Untreated [Control_Rep2] |
| GSM7116306 |
WT cells, 48h activated in vitro; IL-12 [IL-12_Rep1] |
| GSM7116307 |
WT cells, 48h activated in vitro; IL-12 [IL-12_Rep2] |
| GSM7116308 |
WT cells, 48h activated in vitro; IL-12 + ACBI1 [IL-12 + ACBI1_Rep1] |
| GSM7116309 |
WT cells, 48h activated in vitro; IL-12 + ACBI1 [IL-12 + ACBI1_Rep2] |
| GSM7116310 |
WT cells, 48h activated in vitro; IL-12 + BRM014 [IL-12 + BRM014_Rep1] |
| GSM7116311 |
WT cells, 48h activated in vitro; IL-12 + BRM014 [IL-12 + BRM014_Rep2] |
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| This SubSeries is part of SuperSeries: |
| GSE228381 |
Canonical BAF complex activity shapes the enhancer landscape that licenses CD8+ T cell effector and memory fates |
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| Relations |
| BioProject |
PRJNA983073 |
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