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Series GSE22827 Query DataSets for GSE22827
Status Public on Jul 19, 2010
Title Cell of origin influences molecular and functional properties of murine induced pluripotent stem cells
Organism Mus musculus
Experiment type Methylation profiling by genome tiling array
Summary Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. Here, we show that iPSCs obtained from fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of iPSCs into embryoid bodies (EBs) and different hematopoietic cells. Importantly, continuous passaging of iPSCs largely attenuates these differences. Our results suggest that low-passage iPSCs retain a transient epigenetic memory of their somatic cells of origin, which manifests as differential gene expression and ltered differentiation capacity. These observations might affect ongoing attempts to use iPSCs for disease modeling and also could be exploited for potential therapeutic applications to enhance differentiation into desired cell lineages.

Keywords: DNA methylation profiling
Overall design Direct comparison of DNA methylation in iPS cells derived from different tissues
Contributor(s) Figueroa ME, Polo JM, Melnick A, Hochedlinger K
Citation(s) 20644536
Submission date Jul 08, 2010
Last update date Mar 22, 2012
Contact name Maria Eugenia Figueroa
Organization name University of Miiami
Department Human Genetics
Lab Maria Figueroa
Street address 1501 NW 10th Ave, BRB 709A, Locator code C227
City Miami
State/province FL
ZIP/Postal code 33136
Country USA
Platforms (1)
GPL10283 Nimblegen Mouse 25K MM5_HELP_Promoter array
Samples (18)
GSM564319 Gra-iPSCs_1 P4
GSM564320 Gra-iPSCs_2 P4
GSM564321 Gra-iPSCs_3 P4
BioProject PRJNA127875

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22827_RAW.tar 235.3 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table

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