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Series GSE228301 Query DataSets for GSE228301
Status Public on Sep 27, 2023
Title Progestin receptor promotes cell macropinocytosis through CDC42 in pancreatic cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Endocrine receptors play an essential role in tumor metabolic reprogramming and represent a potential therapeutic approach in pancreatic ductal adenocarcinoma (PDAC). PDAC is characterized by a nutrient-deprived microenvironment, and to support their energetic needs, they can internalize extracellular proteins via macropinocytosis. In this study, we found that progesterone receptor (PGR), a steroid-responsive nuclear receptor, has a higher expression in PDAC tissues from patients and transgenic LSL-KrasG12D/+; LSL-Trp53R172H/+; PDX1-cre (KPC) mice. Moreover, PGR knockdown restrained PDAC cell survival and tumor growth both in vitro and in vivo. Genetic and pharmacological PGR inhibition resulted in dramatically macropinocytosis impairment in PDAC cells and subcutaneous tumor models, which indicates involvement of this receptor in macropinocytosis regulation. Mechanistically, PGR deficiency caused a subsequently decreased expression of CDC42, a key regulator in macropinocytosis. These data deepen our understanding of how endocrine system influences tumor progression via non-classical pathway and provide a novel therapeutic option for patients with PDAC.
 
Overall design The cDNA microarrays were performed to investigate the differentially expressed cDNA in PGR-interference AsPC-1 cells compared to control cells.
 
Contributor(s) Nie H, Li P, Gai Y, Pan H, Liao Y, Qian L, Zhang Y
Citation(s) 38424455
Submission date Mar 27, 2023
Last update date Mar 07, 2024
Contact name yanzhi Gai
E-mail(s) yanzhi_gai@sjtu.edu.cn
Phone 15200895615
Organization name Shanghai Jiao Tong university
Street address 800 Dongchuan Road
City Shanghai
ZIP/Postal code 200001
Country China
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (2)
GSM7118511 As.Ctrl
GSM7118512 As.si.PGR
Relations
BioProject PRJNA949219

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE228301_RAW.tar 9.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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