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Series GSE228361 Query DataSets for GSE228361
Status Public on Mar 03, 2024
Title Landscape of TFs binding in mouse early embryos [HiCuT]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In mammals, early lineage specification originates from the establishment of cell polarization in early embryos, before providing the foundation for all somatic development. Although there have been heated debates about whether transcription factors (TFs) or epigenetic information plays the dominant role in cell fate decisions, the precise annotation of TFs remains largely elusive in early embryos, restricting the functional study of developmentally important TFs. Here, we investigated the genome-wide landscapes of two known triggers of mouse embryo polarization: transcription factor AP-2 gamma (Tfap2c) and TEA domain transcription factor 4 (Tead4). We found that Tfap2c can bridge the connection between the promoter and the proximal enhancer, and further facilitate the functions of downstream Tead4 and Klf5 in boosting polarization. Our study also implicated that Rarg-initiated active demethylation might be a prerequisite for proper Tfap2c functioning. Furthermore, the results demonstrated that both genomic imprinting and nucleotide polymorphism can instruct TF binding, leading to allele-specific expression in early embryos. Overall, our study characterized TF dynamics during the peri-implantation stage and revealed their spatiotemporal interactions with the epigenetic environment in driving polarization and lineage specification.
 
Overall design HiCuT experiment on E9.5 placenta, mice crossed by c57(m) x pwk(p)
 
Contributor(s) Gao R, Yang G
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 28, 2023
Last update date Mar 04, 2024
Contact name Gran Young
Organization name Tongji University
Street address 1239th, Siping Road, Yangpu district
City Shanghai
State/province --- Select a state ---
ZIP/Postal code 200082
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (1)
GSM7119414 HiCuT, E9.5, placenta, c57(m) x pwk(p)
This SubSeries is part of SuperSeries:
GSE212568 Landscape of TFs binding in mouse early embryos
Relations
BioProject PRJNA949561

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE228361_RAW.tar 4.0 Mb (http)(custom) TAR (of BEDPE)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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