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Series GSE228546 Query DataSets for GSE228546
Status Public on Jun 13, 2023
Title Effect of BAF disruption on T-bet binding in activated CD8+ T cells [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To investigate the role of cBAF in regulation of Tbet binding, polyclonal naive CD8 T cells were stimulated for 48h with anti-CD3, CD28, and hIL-2.
Cells were then treated with DMSO, ACBI1, or BRM014 for 6 hours, and treated with recombinant mouse IL-12 for the last two hours of inhibitor treatment. ChIP-seq against T-bet was then performed.
Overall design T-bet ChIP-seq on WT CD8+ T cells activated with anti-CD3, anti-CD28, and hIL-2 for 48h in vitro with or without IL-12, ACBI-1, or BRM014.
Contributor(s) McDonald B, Chick B, Ahmed N
Citation(s) 37315534
Submission date Mar 30, 2023
Last update date Sep 12, 2023
Contact name Bryan McDonald
Organization name Salk Institute for Biological Studies
Street address 10010 N Torrey Pines Rd
City San Diego
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL30172 NextSeq 2000 (Mus musculus)
Samples (8)
GSM7123802 WT cells, 48h activated in vitro; Untreated, ChIP
GSM7123803 WT cells, 48h activated in vitro; IL-12, ChIP
GSM7123804 WT cells, 48h activated in vitro; IL-12 + ACBI1, ChIP
This SubSeries is part of SuperSeries:
GSE228381 Canonical BAF complex activity shapes the enhancer landscape that licenses CD8+ T cell effector and memory fates
BioProject PRJNA950325

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Supplementary file Size Download File type/resource
GSE228546_RAW.tar 885.3 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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