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Series GSE228546 Query DataSets for GSE228546
Status Public on Jun 13, 2023
Title Effect of BAF disruption on T-bet binding in activated CD8+ T cells [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To investigate the role of cBAF in regulation of Tbet binding, polyclonal naive CD8 T cells were stimulated for 48h with anti-CD3, CD28, and hIL-2.
Cells were then treated with DMSO, ACBI1, or BRM014 for 6 hours, and treated with recombinant mouse IL-12 for the last two hours of inhibitor treatment. ChIP-seq against T-bet was then performed.
 
Overall design T-bet ChIP-seq on WT CD8+ T cells activated with anti-CD3, anti-CD28, and hIL-2 for 48h in vitro with or without IL-12, ACBI-1, or BRM014.
 
Contributor(s) McDonald B, Chick B, Ahmed N
Citation(s) 37315534
Submission date Mar 30, 2023
Last update date Sep 12, 2023
Contact name Bryan McDonald
E-mail(s) bmcdonald@salk.edu
Organization name Salk Institute for Biological Studies
Street address 10010 N Torrey Pines Rd
City San Diego
State/province CA
ZIP/Postal code 92037
Country USA
 
Platforms (1)
GPL30172 NextSeq 2000 (Mus musculus)
Samples (8)
GSM7123802 WT cells, 48h activated in vitro; Untreated, ChIP
GSM7123803 WT cells, 48h activated in vitro; IL-12, ChIP
GSM7123804 WT cells, 48h activated in vitro; IL-12 + ACBI1, ChIP
This SubSeries is part of SuperSeries:
GSE228381 Canonical BAF complex activity shapes the enhancer landscape that licenses CD8+ T cell effector and memory fates
Relations
BioProject PRJNA950325

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE228546_RAW.tar 885.3 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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