|Public on Jun 13, 2023
|RIF1 regulates early replication timing in murine B cells.
|Genome binding/occupancy profiling by high throughput sequencing
|The mammalian DNA replication timing (RT) program is crucial for the proper functioning and integrity of the genome. The best-known mechanism for controlling RT is the suppression of late origins of replication in heterochromatin by RIF1. Here, we report that in antigen-activated, hypermutating murine B lymphocytes, RIF1 binds predominantly to early-replicating active chromatin and promotes early replication, but plays a minor role in regulating replication origin activity, gene expression and genome organization in B cells. Furthermore, we find that RIF1 functions in a complementary and non-epistatic manner with minichromosome maintenance (MCM) proteins to establish early RT signatures genome-wide and, specifically, to ensure the early replication of highly transcribed genes. These findings reveal new layers of regulation within the B cell RT program, driven by the coordinated activity of RIF1 and MCM proteins.
|Refer to individual Series
|Apr 03, 2023
|Last update date
|Dec 14, 2023
|Illumina HiSeq 2500 (Mus musculus)
|NextSeq 550 (Mus musculus)
|Illumina NovaSeq 6000 (Mus musculus)
|This SuperSeries is composed of the following SubSeries:
|RIF1 regulates replication origin activity and early replication timing in B cells [ChIP-seq]
|RIF1 regulates replication origin activity and early replication timing in B cells [HI-C]
|RIF1 regulates replication origin activity and early replication timing in B cells [PRO-seq]