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Series GSE228880 Query DataSets for GSE228880
Status Public on Jun 13, 2023
Title RIF1 regulates early replication timing in murine B cells.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The mammalian DNA replication timing (RT) program is crucial for the proper functioning and integrity of the genome. The best-known mechanism for controlling RT is the suppression of late origins of replication in heterochromatin by RIF1. Here, we report that in antigen-activated, hypermutating murine B lymphocytes, RIF1 binds predominantly to early-replicating active chromatin and promotes early replication, but plays a minor role in regulating replication origin activity, gene expression and genome organization in B cells. Furthermore, we find that RIF1 functions in a complementary and non-epistatic manner with minichromosome maintenance (MCM) proteins to establish early RT signatures genome-wide and, specifically, to ensure the early replication of highly transcribed genes. These findings reveal new layers of regulation within the B cell RT program, driven by the coordinated activity of RIF1 and MCM proteins.
Overall design Refer to individual Series
Web link
Citation(s) 38081811
Submission date Apr 03, 2023
Last update date Dec 14, 2023
Contact name Tobias Neumann
Organization name IMP
Street address Campus-Vienna-Biocenter 1
City Vienna
ZIP/Postal code 1030
Country Austria
Platforms (4)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21626 NextSeq 550 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (69)
GSM7140982 priB_Rif1FH_ChIPseq_rep1
GSM7140983 priB_Rif1FH_ChIPseq_rep2
GSM7140984 priB_Rif1WT_ChIPseq_rep1
This SuperSeries is composed of the following SubSeries:
GSE228875 RIF1 regulates replication origin activity and early replication timing in B cells [ChIP-seq]
GSE228876 RIF1 regulates replication origin activity and early replication timing in B cells [HI-C]
GSE228877 RIF1 regulates replication origin activity and early replication timing in B cells [PRO-seq]
BioProject PRJNA951767

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Supplementary file Size Download File type/resource
GSE228880_RAW.tar 6.2 Gb (http)(custom) TAR (of BEDGRAPH, BW, HIC, MCOOL)
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