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Series GSE229153 Query DataSets for GSE229153
Status Public on May 05, 2023
Title Hexokinase 2 and lactate mediated gene expression via histone H3 lysine 18 lactylation (CHO RNA-Seq)
Organism Cricetulus griseus
Experiment type Expression profiling by high throughput sequencing
Summary Hexokinase catalyzes the first committed step in glucose metabolism by phosphorylating glucose to produce glucose-6-phosphate. Highly glycolytic proliferating cells such as cancer cells take advantage of HK2 expression to accelerate glucose metabolism even in the presence of oxygen. This acceleration not only provides sufficient glycolytic intermediates to support the anabolic demands of the cells but also inevitably accompanies increased formation of metabolic end products such as lactate. Currently, the effect of lactate caused by HK2-mediated metabolic alteration is largely unknown. A recent study found that lactate plays a role in an epigenetic alteration known as histone lactylation. Here, using RNA-seq and CUT&Tag chromatin profiling, we study the effect of HK2 and lactate on gene expression via histone lactylation (H3K18la).
 
Overall design Comparative gene expression profiling analysis of RNA-seq data for hexokinase null (EV), wildtype HK2 (WTHK2) expressing cells, and EV with 10 mM lactate treatment (LAC) for 24 h.
 
Contributor(s) Hay N, Rho H
Citation(s) 37463576
Submission date Apr 06, 2023
Last update date Aug 04, 2023
Contact name Nissim Hay
E-mail(s) nhay@uic.edu
Organization name University of Illinois Chicago
Department BMG
Street address 900 South Ashland Ave
City Chicago
State/province IL
ZIP/Postal code 60607
Country USA
 
Platforms (1)
GPL27425 Illumina NovaSeq 6000 (Cricetulus griseus)
Samples (9)
GSM7156045 CHO_EV_rep1
GSM7156046 CHO_EV_rep2
GSM7156047 CHO_EV_rep3
This SubSeries is part of SuperSeries:
GSE229156 Hexokinase 2 and lactate
Relations
BioProject PRJNA953054

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE229153_CHO_RNAseq_NormCPM.txt.gz 355.5 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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