GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE229259 Query DataSets for GSE229259
Status Public on May 17, 2024
Title Ambient RNA analysis of cortex-specific single-nuclei RNA-Seq (snRNA-seq) data in the sham and bilateral carotid artery stenosis (BCAS) groups
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary When compared with the sham group, ambient RNAs were more predominant in the BCAS group, which originated from damaged neuronal nuclei. After CellBender and subcluster cleaning treatments, detectable ambient RNA contamination could be largely reduced, and cortex-specific transcriptomic maps in the sham and BCAS groups were generated successfully.
The underlying cellular and molecular mechanisms related to cortex damage and glial activation after severe cerebral hypoperfusion were further investigated at single nuclei resolution.
Overall design Cortex-specific gene expression profiling analysis at single-nuclei resolution was performed using data obtained from RNA-seq in sham and BCAS mice
Contributor(s) Zhang Y
Citation(s) 37917300
Submission date Apr 08, 2023
Last update date May 18, 2024
Contact name Yuan Zhang
Organization name Fudan University
Street address 130 Dong an Road, Shanghai 200032, China
City Shanghai
State/province Shanghai
ZIP/Postal code 200032
Country China
Platforms (1)
GPL28330 DNBSEQ-T7 (Mus musculus)
Samples (4)
GSM7157739 snRNA_seq_Sham_1
GSM7157740 snRNA_seq_Sham_2
GSM7157741 snRNA_seq_BCAS_1
BioProject PRJNA953658

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE229259_RAW.tar 472.3 Mb (http)(custom) TAR (of H5)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap