GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE229311 Query DataSets for GSE229311
Status Public on Jun 07, 2023
Title Single Cell Transcriptomics of Bone Marrow Derived Macrophages reveal Ccl5 as a marker of Direct IFNAR-independent Responses to DNA Sensing
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Bone marrow derived macrophages (BMDMs) are a common model for macrophage type 1 interferon responses. We used single cell RNA-seq (scRNA-seq) to identify direct responders for dsDNA sensing.
Overall design Bone marrow cells were isolated from C57BL/6J wild-type and IFNAR -/- mice. The cells were treated with macrophage colony-stimulating factor (M-CSF) and then stimulated with control vehicle, dsDNA, or dsDNA and IFNAR antibody for analysis with scRNA-seq.
Contributor(s) King KR, McCarty E, Yu J, Ninh VK, Calcagno DM, Lee J
Citation(s) 37275883
Submission date Apr 10, 2023
Last update date Jun 08, 2023
Contact name Kevin Robert King
Phone 4805595759
Organization name University of California San Diego
Department Bioengineering; Medicine
Street address 9500 Gilman Dr
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (8)
GSM7158692 IFNAR 1 - Wt Control Macrophages, 2 biol replicates (cell culture), scRNA-seq
GSM7158693 IFNAR 2 - Wt Macrophages dsDNA, 2 biol replicates (cell culture), scRNA-seq
GSM7158694 IFNAR 3 - Wt Macrophages, dsDNA+IFNARab, 2 biol replicates (cell culture), scRNA-seq
BioProject PRJNA953992

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE229311_RAW.tar 389.9 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap