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GEO help: Mouse over screen elements for information. |
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Status |
Public on Jan 01, 2011 |
Title |
PFOS and PFHxS decrease lipoprotein production |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array Third-party reanalysis
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Summary |
The effect of perfluoroalkyl sulfonates on lipoprotein metabolism was investigated in APOE*3-Leiden.CETP mice with a humanized lipoprotein profile. Perfluorohexane sulfonate and perfluorooctane sulfonate markedly reduced both plasma TG and TC by decreasing nonHDL-C and HDL-C accompanied by a reduction in apoAI. Mechanistic studies showed that these effects were mainly caused by impaired lipoprotein production.
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Overall design |
Male E3L.CETP mice on a C57Bl/6 background were fed a Western-type diet, containing 0.25% (w/w) cholesterol, 1% (w/w) corn oil and 14% (w/w) bovine fat (Western-type diet) (Hope Farms, Woerden, The Netherlands) for 4 to 6 weeks in three independent experiments. Upon randomization according to total plasma cholesterol (TC) and TG levels, mice received the Western-type diet without or with PFBS (30 mg/kg/day), PFHxS (6 mg/kg/day) or PFOS (3 mg/kg/day) during 4-6 weeks. The effects of these PFAS on plasma lipids and lipoproteins were determined. In addition, PFAS levels were determined in plasma and livers were isolated for hepatic lipid analysis and hepatic gene expression analysis using an Affymetrix technology platform and Affymetrix GeneChip® mouse genome 430 2.0 arrays.
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Contributor(s) |
Bijland S, Rensen PC, Pieterman EJ, Maas AC, van der Hoorn JW, van Erk MJ, Havekes LM, Willems van Dijk K, Chang S, Ehresman DJ, Butenhoff JL, Princen HM |
Citation(s) |
21705711 |
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Submission date |
Jul 14, 2010 |
Last update date |
Jan 17, 2013 |
Contact name |
MJ van Erk |
E-mail(s) |
marjan.vanerk@tno.nl
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Organization name |
TNO Quality of Life
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Street address |
PO Box 360
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City |
Zeist |
ZIP/Postal code |
3700AJ |
Country |
Netherlands |
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Platforms (1) |
GPL10288 |
Affymetrix GeneChip Mouse Genome 430 2.0 Array [CDF: Mm_ENTREZG_11] |
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Samples (18)
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Relations |
Affiliated with |
GSM530347 |
Affiliated with |
GSM530348 |
Affiliated with |
GSM530349 |
Affiliated with |
GSM530350 |
Affiliated with |
GSM530351 |
Affiliated with |
GSM530352 |
Affiliated with |
GSM530353 |
Affiliated with |
GSM530354 |
Affiliated with |
GSM530355 |
Affiliated with |
GSM530356 |
Affiliated with |
GSM530357 |
Affiliated with |
GSM530358 |
BioProject |
PRJNA127779 |
Supplementary file |
Size |
Download |
File type/resource |
GSE22940_RAW.tar |
68.5 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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