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| Status |
Public on Apr 30, 2023 |
| Title |
Single Nucleus RNA sequencing of the mouse placental junctional zone |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To characterise the transcriptome of mouse placental junctional zone cells in Control litters (deletion of the maternally-inherited and silent Igf2 allele, with normal Igf2 levels in whole litters) in UE litters (dams carried offspring with a deletion of the paternally-inherited Igf2 allele in the placental endocrine cells with diminished Igf2 production from the endocrine cells in whole litters), we performed single nucleus RNA sequencing on the placenta Junctional zone 8 Control placentas (offspring sex: 4M:4F) and 8 UE placentas (offspring sex: 4M:4F) in 2 batches each.
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| Overall design |
The placental junctional zone was dissected from 8 Control and 8 UE litters and stored at -80ºC. Single nucleus suspensions were made, pooling together 2x 4 junctional zones from Control litters (offspring sex: 2M, 2F) and 2x 4 junctional zones from UE litters (offspring sex: 2M, 2F), totalling 4 different samples. Suspensions were FACS sorted for DRAQ5 positive events. Single nucleus RNA sequencing libraries were generated using the 10X Chromium single cell 3' reagents.
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| Contributor(s) |
Lopez-Tello J, Sandovici I, Dowsett GK, Yeo GS, Lam BY, Constancia M, Sferuzzi-Perri AN |
| Citation(s) |
37437545 |
| |
| Submission date |
Apr 12, 2023 |
| Last update date |
Jul 31, 2023 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
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| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
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| Street address |
Box 289 Addenbookes Hospital, Hills Road
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA954811 |
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