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Series GSE22957 Query DataSets for GSE22957
Status Public on Sep 21, 2010
Title Genome-wide expression analysis in kyp and cmt3 mutants of Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Investigation of genome-wide expression in the mutant of histone H3K9 methyltransferase KRYPTONITE (KYP) or DNA methyltransferase CHROMOMETHYLASE3 (CMT3) in Arabidopsis. These mutants showed decrease in H3K9 methylation and DNA methylation levels, and transcriptional activation at transposons and repeats. Using NimbleGen DNA microarray, global pattern of expression of genes and transposons were examined in these mutants.
Overall design Total RNA from leaves was isolated using the RNeasy Plant Mini kit (Qiagen) and was treated with DNase I (TAKARA). Double-stranded cDNA was synthesized using the SuperScript Double Stranded cDNA Synthesis kit (Invitrogen) and oligo (dT)20 primer. cDNA was labeled by Cy3, hybridized to 4 x 72k array, and scanned according to manufacture's instructions (
Contributor(s) Inagaki S, Kakutani T
Citation(s) 20834229
Submission date Jul 15, 2010
Last update date Jan 02, 2015
Contact name Soichi Inagaki
Organization name Nara Institute of Science and Technology
Department Graduate School of Biological Sciences
Street address 8916-5, Takayama-cho
City Ikoma
State/province Nara
ZIP/Postal code 630-0192
Country Japan
Platforms (1)
GPL10689 NimbleGen Arabidopsis thaliana 4x72K Array
Samples (6)
GSM566754 wild type Col-0_1
GSM566755 wild type Col-0_2
GSM566756 kyp_1
This SubSeries is part of SuperSeries:
GSE23030 Autocatalytic differentiation of epigenetic modifications within the Arabidopsis genome
BioProject PRJNA133095

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22957_RAW.tar 23.6 Mb (http)(custom) TAR (of CALLS, PAIR)
Processed data included within Sample table
Processed data provided as supplementary file

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