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Series GSE229970 Query DataSets for GSE229970
Status Public on Nov 12, 2023
Title Unraveling the diversity and functions of tissue-resident plasma cells (RNA-seq of mouse antibody-secreting cells)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Antibody-secreting plasma cells (PCs) are generated in secondary lymphoid organs, but are reported to reside in an emerging range of anatomical sites. Analysis of the transcriptome of different tissue-resident (Tr)PC populations revealed that they each have their own transcriptional signature indicative of functional adaptation to the host tissue environment. In contrast to expectation, all TrPCs were extremely long-lived, regardless of their organ of residence, with the longevity influenced by intrinsic factors like the immunoglobulin isotype. This study reveals that extreme longevity is an intrinsic property of TrPCs whose transcriptome is imprinted by signals received both at the site of induction and within the tissue of residence.
Overall design RNA-seq gene expression profiling analysis was performed on tissue-resident antibody-secreting cells (plasma cells and plasmablasts. Ex vivo cells were sorted by Blimp1-gfp+ CD138+ or Blimp1-gfp+ CD98+. Plasma cells were isolated from bone marrow (1 biological replicate), colon (3 replicates), small intestine (3 replicates), mammary gland (3 replicates), Peyer’s patches (2 replicates) and spleen (2 replicates). Plasmablasts were isolated from Peyer's patches (2 replicates) and spleen (2 replicates). Bone marrow plasma cells were further sorted into IgA and non-IgA isotypes (3 biological replicates).
Contributor(s) Tarasova I, Smyth GK, Tellier J, Nutt SL
Citation(s) 38172260
Submission date Apr 18, 2023
Last update date Jan 11, 2024
Contact name Gordon K Smyth
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (24)
GSM7182083 Bone marrow IgA plasma cells, biol rep 1
GSM7182084 Bone marrow IgA plasma cells, biol rep 2
GSM7182085 Bone marrow non-IgA plasma cells, biol rep 1
BioProject PRJNA956912

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE229970_GenewiseCounts.txt.gz 796.5 Kb (ftp)(http) TXT
GSE229970_RAW.tar 4.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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