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Series GSE230284 Query DataSets for GSE230284
Status Public on May 15, 2024
Title yHSC and oHSC 10X scRNAseq under ad lib, fasted, or fast refeed conditions to evaluate transcriptional impact of transient autophagy engagement in hematopoietic stem cells.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We observed that 24hr of fasting followed by a 24hr refeeding period improved the regenerative capacity of hematopoietic stem cells isolated from old mice, which was associated with an increase in glycolytic capacity. We sought to evaluate transcriptional changes in yHSC and oHSC compartment following different mouse feeding paradigms at single cell resolution. In yHSCs, AL and F/R groups showed overlapping transcriptional signatures with a distinct pattern for the fasted group. In contrast, in oHSCs, AL and fasted groups showed a strong overlap with a distinct transcriptional signature for the F/R group, reflective of differences in metabolic set points with age.
Overall design Three young and three old mice were analyzied (one per feeding condition - Ad lib, 24hr Fast, 24hr Fast 24hr Refeed)
Contributor(s) Dellorusso P, Passegue E
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Submission date Apr 21, 2023
Last update date May 15, 2024
Contact name Paul Vincent Dellorusso
Phone 5166552491
Organization name Columbia University Irving Medical Center
Department Genetics and Development
Lab Passegue Lab
Street address 701 West 168th Street, 724 Hammer Health Sciences Center
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM7211267 young ad libidum
GSM7211268 young fasted
GSM7211269 young fasted/refed
This SubSeries is part of SuperSeries:
GSE229137 Autophagy engaging and non-engaging hematopoietic stem cells
BioProject PRJNA958196

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE230284_c0vsRest_DEtable.txt.gz 724.4 Kb (ftp)(http) TXT
GSE230284_c0vsRest_DEtable_c2Pathway.txt.gz 131.5 Kb (ftp)(http) TXT
GSE230284_c10vsRest_DEtable.txt.gz 695.5 Kb (ftp)(http) TXT
GSE230284_c10vsRest_DEtable_c2Pathway.txt.gz 121.1 Kb (ftp)(http) TXT
GSE230284_c1vsRest_DEtable.txt.gz 702.5 Kb (ftp)(http) TXT
GSE230284_c1vsRest_DEtable_c2Pathway.txt.gz 129.9 Kb (ftp)(http) TXT
GSE230284_c2vsRest_DEtable.txt.gz 715.5 Kb (ftp)(http) TXT
GSE230284_c2vsRest_DEtable_c2Pathway.txt.gz 130.0 Kb (ftp)(http) TXT
GSE230284_c3vsRest_DEtable.txt.gz 698.2 Kb (ftp)(http) TXT
GSE230284_c3vsRest_DEtable_c2Pathway.txt.gz 128.3 Kb (ftp)(http) TXT
GSE230284_c4vsRest_DEtable.txt.gz 703.2 Kb (ftp)(http) TXT
GSE230284_c4vsRest_DEtable_c2Pathway.txt.gz 129.2 Kb (ftp)(http) TXT
GSE230284_c5vsRest_DEtable.txt.gz 664.0 Kb (ftp)(http) TXT
GSE230284_c5vsRest_DEtable_c2Pathway.txt.gz 121.4 Kb (ftp)(http) TXT
GSE230284_c6vsRest_DEtable.txt.gz 709.1 Kb (ftp)(http) TXT
GSE230284_c6vsRest_DEtable_c2Pathway.txt.gz 128.7 Kb (ftp)(http) TXT
GSE230284_c7vsRest_DEtable.txt.gz 707.9 Kb (ftp)(http) TXT
GSE230284_c7vsRest_DEtable_c2Pathway.txt.gz 127.3 Kb (ftp)(http) TXT
GSE230284_c8vsRest_DEtable.txt.gz 701.0 Kb (ftp)(http) TXT
GSE230284_c8vsRest_DEtable_c2Pathway.txt.gz 126.9 Kb (ftp)(http) TXT
GSE230284_c9vsRest_DEtable.txt.gz 688.2 Kb (ftp)(http) TXT
GSE230284_c9vsRest_DEtable_c2Pathway.txt.gz 123.5 Kb (ftp)(http) TXT
Raw data are available in SRA
Processed data are available on Series record

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