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| Status |
Public on Jun 07, 2023 |
| Title |
Prenatal exposure to benzo[a]pyrene depletes ovarian reserve and masculinizes embryonic ovarian germ cell transcriptome transgenerationally [Bisulfite-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
People are exposed to polycyclic aromatic hydrocarbons, like benzo[a]pyrene (BaP), via inhalation of particulate matter air pollution and ingestion of grilled and smoked foods. Prenatal exposure to BaP destroys germ cells in ovaries, causing earlier onset of ovarian senescence post-natally. Developing testes are affected at higher doses than ovaries. However, it is not known if adverse effects are transmitted to subsequent generations. We orally dosed pregnant female mice (F0) with 0.033, 0.2, or 2 mg/kg-day BaP or vehicle from embryonic day (E) 6.5-11.5 (F1 offspring) or E6.5-15.5 (F2 and F3). Ovarian germ cell and follicle numbers were significantly decreased in F3 females at all doses of BaP; testicular germ cells were not affected. E13.5 germ cell RNA-sequencing revealed significantly increased expression of male-specific genes in female germ cells across generations and BaP doses. Our study demonstrated that F0 BaP exposure partially disrupted sexual identity of female germ cells transgenerationally.
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| Overall design |
Whole genome bisulfite sequencing of FACS-enriched mouse primordial germ cells with or without gestational exposure to banzo[a]pyrene: Repeated Experiment (F3 primordial germ cells)
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| Contributor(s) |
Shioda T, Lim J, Luderer U |
| Citation(s) |
37248279 |
| |
| Submission date |
Apr 22, 2023 |
| Last update date |
Jun 08, 2023 |
| Contact name |
Toshi Shioda |
| E-mail(s) |
tshioda@partners.org
|
| Phone |
(617) 726-3425
|
| Organization name |
MGH Cancer Center
|
| Department |
Center for Cancer Research
|
| Lab |
Molecular Profiling Lab
|
| Street address |
Building 149 - 7th Floor, 13th Street
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA960654 |
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