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Series GSE230414 Query DataSets for GSE230414
Status Public on Jun 07, 2023
Title Molecular signatures of CD45+ cells from brain and spleen after intracerebral hemorrhage
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Leukocyte infiltration accelerates brain injury following intracerebral hemorrhage (ICH). But the involvement of T lymphocytes in this process has not been fully elucidated.To further depict the landscape of the composition and functional states of brain-infiltrating immune cells following ICH, single-cell RNA sequencing was performed to compare the molecular characteristics of CD45+ cells from brain and spleen tissues in ICH and control mice.Our results revealed that brain-infiltrating T cells exhibited enhanced pro-inflammatory and pro-apoptotic signatures.
Overall design CD45+ cells were sorted from the spleen and brain of ICH mice or sham controls at day 3, and were subjected to single-cell RNA sequencing. And to obtain enough CD4+ T cells in sham brains for sub-clustering and data processing, we optimized the FACS-sorting to enrich CD45hi cells. Data were pooled from 15 mice in each group.
Contributor(s) Shi SX, Xiu Y, Li Y, Shi K, Yuan M, Liu Q, Wang X, Jin W
Citation(s) 37285421
Submission date Apr 24, 2023
Last update date Jul 21, 2023
Contact name Wei-Na Jin
Organization name Beijing Tiantan Hospital, Capital Medical University
Department China National Clinical Research Center for Neurological Diseases
Street address NO.119, Southwest of the Fourth Ring Road, Fengtai District
City Beijing
State/province Beijing
ZIP/Postal code 100070
Country China
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL28457 DNBSEQ-G400 (Mus musculus)
Samples (4)
GSM7221680 ICH_Brain
GSM7221681 ICH_Spleen
GSM7221682 Control_Spleen
BioProject PRJNA961095

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Supplementary file Size Download File type/resource
GSE230414_RAW.tar 100.2 Mb (http)(custom) TAR (of TAB)
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Raw data are available in SRA
Processed data provided as supplementary file

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