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| Status |
Public on Jun 07, 2023 |
| Title |
Molecular signatures of CD45+ cells from brain and spleen after intracerebral hemorrhage |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Leukocyte infiltration accelerates brain injury following intracerebral hemorrhage (ICH). But the involvement of T lymphocytes in this process has not been fully elucidated.To further depict the landscape of the composition and functional states of brain-infiltrating immune cells following ICH, single-cell RNA sequencing was performed to compare the molecular characteristics of CD45+ cells from brain and spleen tissues in ICH and control mice.Our results revealed that brain-infiltrating T cells exhibited enhanced pro-inflammatory and pro-apoptotic signatures.
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| Overall design |
CD45+ cells were sorted from the spleen and brain of ICH mice or sham controls at day 3, and were subjected to single-cell RNA sequencing. And to obtain enough CD4+ T cells in sham brains for sub-clustering and data processing, we optimized the FACS-sorting to enrich CD45hi cells. Data were pooled from 15 mice in each group.
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| Contributor(s) |
Shi SX, Xiu Y, Li Y, Shi K, Yuan M, Liu Q, Wang X, Jin W |
| Citation(s) |
37285421 |
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| Submission date |
Apr 24, 2023 |
| Last update date |
Jul 21, 2023 |
| Contact name |
Wei-Na Jin |
| Organization name |
Beijing Tiantan Hospital, Capital Medical University
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| Department |
China National Clinical Research Center for Neurological Diseases
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| Street address |
NO.119, Southwest of the Fourth Ring Road, Fengtai District
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100070 |
| Country |
China |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL28457 |
DNBSEQ-G400 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA961095 |
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