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Series GSE230496 Query DataSets for GSE230496
Status Public on Apr 17, 2024
Title Epigenomic profiling by ATAC-seq and ChIL-seq in B cells and plasma cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We performed ATAC-seq and H3.3 ChIL-seq to underdatand dynamic change of chromatin accessibility and H3.3 deposition into genome during B cell differentiation.
 
Overall design ChIL-seq for H3.3 on naive B cells, activated B cells and PCs after LPS stimulation was conducted. ATAC-seq was perfomed in naïve B cells and LPS-induced plamsa cells. We also used B cells expressing GFP-H3.3 or control vector for ATAC-seq and ChiL-seq for anti-H3.3 or GFP antibodies.
 
Contributor(s) Saito Y, Harada A, Ohkawa Y, Baba Y
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Submission date Apr 24, 2023
Last update date Apr 18, 2024
Contact name Yasuyuki Ohkawa
E-mail(s) yohkawa@bioreg.kyushu-u.ac.jp
Organization name Medical Institute of Bioregulation
Lab Division of Transcriptomics
Street address 3-1-1 Maidashi
City Fukuoka
ZIP/Postal code 8128582
Country Japan
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (32)
GSM7225369 Naive, rep1
GSM7225370 Naive, rep2
GSM7225371 Naive, rep3
Relations
BioProject PRJNA961227

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE230496_ATAC_PC_merged.q0.01_summits.bed.gz 770.5 Kb (ftp)(http) BED
GSE230496_ATAC_naive_merged.q0.01_summits.bed.gz 782.4 Kb (ftp)(http) BED
GSE230496_Naive_H3p3_repMerged.q0.01_noMnoL_summits.bed.gz 625.4 Kb (ftp)(http) BED
GSE230496_PC_H3p3_repMerged.q0.01_noMnoL_summits.bed.gz 105.5 Kb (ftp)(http) BED
GSE230496_RAW.tar 1.0 Gb (http)(custom) TAR (of BW)
GSE230496_nonPC_H3p3_repMerged.q0.01_noMnoL_summits.bed.gz 121.0 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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