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Series GSE230530 Query DataSets for GSE230530
Status Public on Jun 07, 2023
Title A gut commensal protist protects against virus-mediated loss of oral tolerance
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To better understand how Tritrichomonas arnold colonization impacts the reovirus-mediated proinflammatory response in dietary antigen-presenting dendtric cells (cDC1), we examined the transcriptional profile of mesenteric lymph node-derived cDC1.
For RNA-sequencing on CD103+ CD11b- CD8a+ DCs, 1,000 CD103+ CD11b- CD8a+ DCs were FACS sorted into a 96-well plate
 
Overall design Wild-type mice were inoculated perorally with 10^6 Tritrichomonas arnold or PBS control for 12 days followed by peroral inoculation with 10^9 PFU of reovirus strain T1L or PBS control for 48 hours. Smart-seq RNA-sequencing of MHC-II+ CD11c+ CD103+ CD11b- CD8a+ mLN DCs (PBS, n =4), (T1L, n = 3), (T1L+T. arnold, n =4), (T. arnold, n = 3).
 
Contributor(s) Hinterleitner R, Jishnu D, Isha MD
Citation(s) 37478853
Submission date Apr 25, 2023
Last update date Sep 06, 2023
Contact name Reinhard Hinterleitner
E-mail(s) REINHARD@pitt.edu
Organization name University of Pittsburgh
Department Immunology
Street address 5051 Centre Avenue
City PITTSBURGH
State/province PA
ZIP/Postal code 15213
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (14)
GSM7226254 DC1, PBS, rep1
GSM7226255 DC1, PBS, rep2
GSM7226256 DC1, PBS, rep3
Relations
BioProject PRJNA961715

Download family Format
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Supplementary file Size Download File type/resource
GSE230530_Table_2_sig_DE_PBS_vs_T1L.xlsx 490.6 Kb (ftp)(http) XLSX
GSE230530_Table_3_sig_DE_T1L_vs_T_arnold+T1L.xlsx 160.0 Kb (ftp)(http) XLSX
GSE230530_Table_4_sig_DE_PBS_vs_T_arnold.xlsx 48.4 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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