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| Status |
Public on Jun 07, 2023 |
| Title |
A gut commensal protist protects against virus-mediated loss of oral tolerance_2 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To better understand how Tritrichomonas arnold colonization impacts the reovirus-mediated proinflammatory response in mesenteric lymph nodes, we examined the transcriptional profile of mesenteric lymph nodes
For RNA-sequencing single cell suspension of mesenteric lymph nodes were lysed in RLT buffer (Qiagen) and RNA was isolated
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| Overall design |
Wild-type mice were inoculated perorally with 10^6 Tritrichomonas arnold or PBS control for 12 days followed by peroral inoculation with 10^9 PFU of reovirus strain T1L or PBS control for 48 hours. Bulk RNA-sequencing mesenteric lymph nodes (PBS, n =4), (T1L, n = 4), (T1L+T. arnold, n =4), (T. arnold, n = 4).
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| Contributor(s) |
Hinterleitner R, Jishnu D, Isha MD |
| Citation(s) |
37478853 |
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| Submission date |
Apr 25, 2023 |
| Last update date |
Sep 06, 2023 |
| Contact name |
Reinhard Hinterleitner |
| E-mail(s) |
REINHARD@pitt.edu
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| Organization name |
University of Pittsburgh
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| Department |
Immunology
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| Street address |
5051 Centre Avenue
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| City |
PITTSBURGH |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (16)
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| GSM7226719 |
mesenteric lymph nodes, PBS, rep4 |
| GSM7226720 |
mesenteric lymph nodes, T. arnold, rep1 |
| GSM7226721 |
mesenteric lymph nodes, T. arnold, rep2 |
| GSM7226722 |
mesenteric lymph nodes, T. arnold, rep3 |
| GSM7226723 |
mesenteric lymph nodes, T. arnold, rep4 |
| GSM7226724 |
mesenteric lymph nodes, T1L, rep1 |
| GSM7226725 |
mesenteric lymph nodes, T1L, rep2 |
| GSM7226726 |
mesenteric lymph nodes, T1L, rep3 |
| GSM7226727 |
mesenteric lymph nodes, T1L, rep4 |
| GSM7226728 |
mesenteric lymph nodes, T1L+T. arnold, rep1 |
| GSM7226729 |
mesenteric lymph nodes, T1L+T. arnold, rep2 |
| GSM7226730 |
mesenteric lymph nodes, T1L+T. arnold, rep3 |
| GSM7226731 |
mesenteric lymph nodes, T1L+T. arnold, rep4 |
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| Relations |
| BioProject |
PRJNA961762 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE230558_Table_1_normalized_counts_genenames.xlsx |
19.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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