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Series GSE230607 Query DataSets for GSE230607
Status Public on Apr 30, 2023
Title The KEAP1-NRF2 pathway regulates TFEB/TFE3-dependent lysosomal biogenesis [RNA-seq: 7dpf_larvae]
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary Under homeostatic conditions, NRF2 is repressed by Kelch-like ECH-associated protein 1 (KEAP1). Here, we demonstrate that Keap1 deficiency induces Nrf2 activation and post-developmental lethality in zebrafish larvae. Loss of viability is preceded by severe liver abnormalities characterized by an accumulation of lysosomes. Mechanistically, we demonstrate that loss of Keap1 promotes aberrant activation of transcription factor EB (TFEB)/transcription factor binding to IGHM Enhancer 3 (TFE3)-dependent lysosomal biogenesis. Importantly, we find that NRF2-dependent regulation of lysosomal biogenesis is cell autonomous and evolutionarily conserved.
Overall design Zebrafish embryos were injected with CRISPR/Cas9 targetting genes at the single-cell stage. RNA was extracted and sequenced at 7dpf.
Contributor(s) Ong AJ, Brown K, Cox A
Citation(s) 37216554
Submission date Apr 26, 2023
Last update date Jul 30, 2023
Contact name Andrew Cox
Phone 0402429160
Organization name PeterMacCallum Cancer Centre
Street address 305 Grattan St
City Melbourne
State/province Victoria
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL20828 Illumina NextSeq 500 (Danio rerio)
Samples (32)
GSM7229784 Bach1 DKO-1
GSM7229785 Bach1 DKO-2
GSM7229786 Bach1 DKO-3
This SubSeries is part of SuperSeries:
GSE230611 The KEAP1-NRF2 pathway regulates TFEB/TFE3-dependent lysosomal biogenesis
BioProject PRJNA961973

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE230607_AO_1.csv.gz 649.0 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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