GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE23080 Query DataSets for GSE23080
Status Public on Jul 24, 2010
Title ChIP-Seq of Histone Methylation and RNAP2 in Human Jurkat cells.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Three transcriptional states can be defined by histone modifications and RNA polymerase II enriched at promoters and across the body of genes. To gain insight into the active, poised and silent genes in human T-ALL cells, two antibodies against RNAP2, and antibodies against H3K4me3, H3K79me2, and H3K27me3 were used for chromatin immunoprecipitation coupled with massive parallel sequencing (ChIP-seq).
Overall design Genomic DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. ChIP was performed using an antibody against RNAP2, H3K4me3, H3K79me2, and H3K27me3 using whole cell extract (WCE) as a background control. ChIP was performed using a two antibody against hypophosphorylated forms of RNAP2 in two biological replicates. All other ChIPs were done in biological replicates with a single lane of sequencing.
Contributor(s) Lawton LN, Young RA, Fan Z
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jul 22, 2010
Last update date May 15, 2019
Contact name Richard A Young
Phone 617-258-5219
Organization name Whitehead Institute for Biomedical Research
Lab Young Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (6)
GSM569085 H3K4m3 ChipSeq Jurkat
GSM569086 WCE_Jurkat
GSM569087 RNAP2 Jurkat_ChipSeq Rpb1 N-terminus
SRA SRP003025
BioProject PRJNA131693

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23080_RAW.tar 152.2 Mb (http)(custom) TAR (of TXT, WIG)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap