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Series GSE230849 Query DataSets for GSE230849
Status Public on Dec 02, 2023
Title Understanding the cell fate and behavior of progenitors at the origin of the mouse cardiac mitral valve
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Congenital heart malformations include mitral valve defects, which remain largely unexplained. During embryogenesis, a restricted population of endocardial cells within the atrioventricular canal undergoes an endothelial-to-mesenchymal transition to give rise to mitral valvular cells. However, the identity and fate decisions of these progenitors as well as the behavior and distribution of their derivatives in valve leaflets remain unknown. We used single-cell RNA sequencing (scRNA-seq) of genetically labeled endocardial cells and microdissected mouse embryonic and postnatal mitral valves to characterize the developmental road. We defined the metabolic processes underlying the specification of the progenitors and their contributions to subtypes of valvular cells. Using retrospective multicolor clonal analysis, we describe specific modes of growth and behavior of endocardial cell-derived clones, which build up, in a proper manner, functional valve leaflets. Our data identify how both genetic and metabolic mechanisms specifically drive the fate of a subset of endocardial cells toward their distinct clonal contribution to the formation of the valve.
Overall design Cells were isolated from enbryos from Tie2crex Rosa26 tdTomato female mice after microdissection of the AVC and embryonic mitral valves. At least 60 embryos were used for each stage of development Cells were then FACS sorted using tomato as a reporter and used in 10X chromium
Contributor(s) Farhat B, Bordeu I, Jagla B, Blanc H, Loulier K, Simons BD, Beaurepaire E, Livet J, Pucéat M
Citation(s) 38198889
Submission date Apr 28, 2023
Last update date Mar 03, 2024
Contact name michel PUCEAT
Phone 33 4 91 32 49 47
Organization name INSERM
Department University aix marseille
Lab physiopathology of cardiac development
Street address 27 bd Jean moulin
City Marseille
ZIP/Postal code 13885
Country France
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM7245158 AVCE9,5
GSM7245159 MVE16,5
GSM7245160 MVNEO1
BioProject PRJNA962839

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Supplementary file Size Download File type/resource
GSE230849_RAW.tar 25.1 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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