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| Status |
Public on May 05, 2023 |
| Title |
Mammalian Genomic Manipulation with Orthogonal Bxb1 DNA Recombinase Sites for the Functional Characterization of Protein Variants |
| Organism |
synthetic construct |
| Experiment type |
Other
|
| Summary |
The Bxb1 bacteriophage serine DNA recombinase is an efficient tool for engineering recombinant DNA into the genomes of cultured cells. Generally, a single engineered “landing pad” site is introduced into the cell genome, permitting the integration of transgenic circuits or libraries of transgene variants. While sufficient for many studies, the extent of genetic manipulation possible with a single recombinase site is limiting, and insufficient for more complex cell-based assays for protein function. Here, we harnessed two orthogonal Bxb1 recombinase sites to enable new avenues for mammalian synthetic biology. By designing plasmids with two recombinase sites, we demonstrate that we can avoid genomic integration of undesirable bacterial DNA elements. We also created “double landing pad” cells simultaneously harboring two orthogonal Bxb1 recombinase sites. These cells allow transgenic protein variant libraries to be readily paired with assay-specific protein partners or biosensors, opening up new functional readouts for large-scale functional assays.
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| Overall design |
Genomically engineered HEK293T cells with amplicons derived from exogenous DNA that has recombined using the Bxb1 bacteriophage integrase and various complementary Bxb1 attP and attB sites
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| |
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| Contributor(s) |
Matreyek KA, Roelle SM |
| Citation(s) |
37922210 |
| |
| Submission date |
Apr 28, 2023 |
| Last update date |
Nov 04, 2023 |
| Contact name |
Kenneth Matreyek |
| E-mail(s) |
Kenneth.Matreyek@Case.edu
|
| Organization name |
Case Western Reserve University
|
| Department |
Pathology
|
| Lab |
Matreyek
|
| Street address |
2103 Cornell Rd
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17769 |
Illumina MiSeq (synthetic construct) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA962992 |
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