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| Status |
Public on Jan 01, 2012 |
| Title |
ChIP-on-chip experiment from Ramos cells to analyze genome-wide CRTC2 binding sites in germinal center B cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
ChIP-on-chip experiment from Ramos cells to analyze genome-wide CRTC2 binding sites in germinal center B cells. The goal was to combine information from our ChIP-on-chip and expression array analyses to compile a list of CRTC2 target genes.
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| Overall design |
CRTC2 was immunoprecipitated from sheared chromatin isolated from untreated Ramos cells using 2 antibodies which recognize distinct epitopes on the CRTC2 protein. Immunoprecipitates were compared to total input chromatin. 2 biological replicates were performed for each antibody.
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| Contributor(s) |
Sherman MH, Deshpande C |
| Citation(s) |
20864035 |
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| Submission date |
Jul 26, 2010 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Chetan Deshpande |
| E-mail(s) |
chetand@amgen.com
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| Organization name |
Amgen Inc
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| Department |
Molecular Sciences
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| Street address |
One Amgen Center Dr
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| City |
Newbury Park |
| State/province |
CA |
| ZIP/Postal code |
91320 |
| Country |
USA |
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| Platforms (2) |
| GPL4124 |
Agilent-014706 Human Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 G4489A (Feature Number version) |
| GPL4125 |
Agilent-014707 Human Promoter ChIP-on-Chip Set 244K, Microarray 2 of 2 G4489A (Feature Number version) |
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| Samples (8)
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| GSM570353 |
Immunoprecipitated chromatin, antibody 1, replicate 1 Set 1 |
| GSM570354 |
Immunoprecipitated chromatin, antibody 1, replicate 1 Set 2 |
| GSM570355 |
Immunoprecipitated chromatin, antibody 1, replicate 2 Set 1 |
| GSM570356 |
Immunoprecipitated chromatin, antibody 1, replicate 2 Set 2 |
| GSM570357 |
Immunoprecipitated chromatin, antibody 2, replicate 1 Set 1 |
| GSM570358 |
Immunoprecipitated chromatin, antibody 2, replicate 1 Set 2 |
| GSM570359 |
Immunoprecipitated chromatin, antibody 2, replicate 2 Set 1 |
| GSM570360 |
Immunoprecipitated chromatin, antibody 2, replicate 2 Set 2 |
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| This SubSeries is part of SuperSeries: |
| GSE23171 |
CRTC2 couples genotoxic stress and plasma cell differentiation in the germinal center |
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| Relations |
| BioProject |
PRJNA133267 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23170_ChIP_Processed_zscore.txt.gz |
11.7 Mb |
(ftp)(http) |
TXT |
| GSE23170_RAW.tar |
545.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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