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Series GSE231931 Query DataSets for GSE231931
Status Public on May 15, 2024
Title RNA-seq analysis of B cells cultured with LPS and IL-12 and/or IFN gamma
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: We aimed to find genes differentially regulated by IL-12 and IFNg alone, and in combination, over 4 and 24 hours of stimulation.
Methods : Spleens were processed and B cell cultures prepared, in triplicate, according to the manuscript. After the indicated times, cells were washed in PBS, resuspended in 350 ul RLT Plus Buffer (Qiagen) with 1% 2-Me, and frozen at -80 ⁰C.
Results: At 4 hours, the main effect of IL-12 is to induce IFNg production from B cells, whereas IFNg induces many gene changes. Comparing 24 hours to the 4 hour stimulation, IFNg alone was insufficient to maintain the majority of IFNg-induced 4 hour changes, while adding IL-12 sustained a portion of the 4 hour gene changes. Additionally, in combination IL-12 and IFNg cooperate to induce unique gene changes that are not shared with either the individual cytokines at either timepoint.
Overall design We used recombinant IL-12 and IFNg or anti-IL-12 and anti-IFNg blocking antibodies to assess the contribution of each cytokine alone and in combination.
Contributor(s) Elsner RA, Smita S, Shlomchik MJ
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Submission date May 08, 2023
Last update date May 15, 2024
Contact name Mark Shlomchik
Organization name University of Pittsburgh
Department Department of Immunology
Street address The Assembly, 5051 Centre Avenue
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
Platforms (1)
GPL30172 NextSeq 2000 (Mus musculus)
Samples (42)
GSM7306190 4 hours, IL-12 at 2000 pg/mL, IFNg block, mouse 1
GSM7306191 4 hours, IL-12 at 2000 pg/mL, IFNg block, mouse 2
GSM7306192 4 hours, IL-12 at 2000 pg/mL, IFNg block, mouse 3
BioProject PRJNA970259

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Supplementary file Size Download File type/resource
GSE231931_RAW.tar 3.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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