NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE232361 Query DataSets for GSE232361
Status Public on Apr 08, 2024
Title MYCT1 Controls Environmental Sensing in human Hematopoietic Stem Cells [scRNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We identified a novel HSC regulatory gene, MYCT1 (MYC target 1), that is selectively expressed in undifferentiated human HSPC and is critical for human HSC expansion and engraftment. MYCT1 knockdown (KD) in human fetal liver and cord blood (CB) HSPCs impaired expansion ex vivo and engraftment after transplantation, whereas restoring the compromised MYCT1 expression via lentiviral overexpression (OE) in cultured human CB HSPCs improved ex vivo expansion of the most undifferentiated human HSPCs (CD34+CD38-CD90+CD45RA-EPCR+ITGA3+) and enhanced their engraftment ability. We performed single cell RNAseq of uncultured human CB HSPCs, as well as 72 hours after MYCT1 KD and OE to identify MYCT1-regulated programs in HSC and understand the role of MYCT1 in governing human HSPC expansion and engraftment ability. Functional enrichment analysis linked the loss of MYCT1 in HLF+ HSCs to a profound dysregulation of multiple cellular programs essential for HSC stemness, such as oxidative phosphorylation or proteostasis, while MYCT1 OE had the opposite effect and showed closer similarity to uncultured HLF+ HSCs. These data indicate that the loss or gain of MYCT1 expression elicits major effects on programs regulating human HSC functional competence.
 
Overall design Single cell RNAseq of sorted human cord blood HSPCs (CD34+CD38-CD90+) either uncultured (day 0) or transduced with control, MYCT1 knockdown, and MYCT1 overexpression lentiviral vectors and resorted (CD34+CD38-CD90+GFP+) 72 hours after transduction (day 4).
 
Contributor(s) Aguadé-Gorgorió J, Ma F, Mikkola HK
Citation(s) 38839950
Submission date May 11, 2023
Last update date Jun 11, 2024
Contact name Júlia Aguadé-Gorgorió
Organization name University of California Los Angeles
Department Molecular, Cell and Developmental Biology
Lab Mikkola lab
Street address 615 Charles E Young Drive South
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM7329389 uncultured, scRNAseq
GSM7329390 transduced control, scRNAseq
GSM7329391 transduced MYCT1 overexpression, scRNAseq
This SubSeries is part of SuperSeries:
GSE232362 MYCT1 Controls Environmental Sensing in human Hematopoietic Stem Cells.
Relations
BioProject PRJNA971733

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE232361_RAW.tar 396.2 Mb (http)(custom) TAR (of MTX, TSV)
GSE232361_aggregatedAll_barcodes.tsv.gz 342.3 Kb (ftp)(http) TSV
GSE232361_aggregatedAll_features.tsv.gz 311.3 Kb (ftp)(http) TSV
GSE232361_aggregatedAll_matrix.mtx.gz 395.9 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap