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Series GSE23252 Query DataSets for GSE23252
Status Public on Aug 05, 2010
Title L2_panneural_tiling_array
Project modENCODE
Organism Caenorhabditis elegans
Experiment type Expression profiling by genome tiling array
Summary modENCODE_submission_462
This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf

Keywords: Transcript tiling array analysis
 
Overall design EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: SD1241 (engineered, target gene F25B3.3 tagged by 3xFlag::PAB-1); Tissue: Pan-neural (L2); Developmental Stage: larva mid-L2 20dC 22h post-L1; Genotype: gaIs153 [(F25B3.3::FLAG::PAB-1) + (sur-5::GFP)]; Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Antibody EZview Red ANTI-FLAGĀ® M2 Affinity Gel (target is FLAG); Strain SD1241 (engineered, target gene F25B3.3 tagged by 3xFlag::PAB-1); temperature 20; Developmental Stage larva mid-L2 20dC 22h post-L1; Tissue Pan-neural (L2)
Web link http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
http://www.modencode.org
 
Contributor(s) Waterston B, Miller D, Gerstein M, Wilson M, Argiwal A, Reinke V
Citation(s) 21177967
BioProject PRJNA168961
Submission date Jul 28, 2010
Last update date May 02, 2013
Contact name DCC modENCODE
E-mail(s) help@modencode.org
Phone 416-673-8579
Organization name Ontario Institute for Cancer Research
Lab modENCODE DCC
Street address MaRS Centre, South Tower, 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
 
Platforms (1)
GPL5634 [Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array
Samples (3)
GSM571437 L2 panneural tiling array extraction1_array1
GSM571438 L2 panneural tiling array extraction2_array1
GSM571439 L2 panneural tiling array extraction3_array1

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23252_L2-panneural_smooth_log2_winsize110.bedgraph.gz 26.3 Mb (ftp)(http) BEDGRAPH
GSE23252_L2-panneural_tars_maxgap30_minrun100_thresh6.42.gff3.gz 1.2 Mb (ftp)(http) GFF3
GSE23252_RAW.tar 114.6 Mb (http)(custom) TAR (of CEL)
Processed data are available on Series record

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