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Series GSE23260 Query DataSets for GSE23260
Status Public on Aug 05, 2010
Title embryo body wall muscle tiling array (v2)
Project modENCODE
Organism Caenorhabditis elegans
Experiment type Expression profiling by genome tiling array
Summary modENCODE_submission_470
This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf

Keywords: Transcript tiling array analysis
 
Overall design EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: PD4251 (engineered, target gene myo-3 tagged by GFP); Tissue: body wall muscle; Developmental Stage: Mixed stage of embryos 20dC; Genotype: ccIs4251 [(myo-3::GFP-LacZ) I ; dpy-20(e1282) IV.]; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain PD4251 (engineered, target gene myo-3 tagged by GFP); temperature 20; Developmental Stage Mixed stage of embryos 20dC; Tissue body wall muscle
Web link http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
http://www.modencode.org
 
Contributor(s) Waterston B, Miller D, Gerstein M, Wilson M, Argiwal A, Reinke V
Citation(s) 21177967
BioProject PRJNA168961
Submission date Jul 28, 2010
Last update date Apr 30, 2013
Contact name DCC modENCODE
E-mail(s) help@modencode.org
Phone 416-673-8579
Organization name Ontario Institute for Cancer Research
Lab modENCODE DCC
Street address MaRS Centre, South Tower, 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
 
Platforms (1)
GPL5634 [Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array
Samples (3)
GSM571461 embryo body wall muscle tiling array (v2) extraction1_array1
GSM571462 embryo body wall muscle tiling array (v2) extraction2_array1
GSM571463 embryo body wall muscle tiling array (v2) extraction3_array1

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23260_RAW.tar 128.3 Mb (http)(custom) TAR (of CEL)
GSE23260_emb-bwm-v2_smooth_log2_winsize110.bedgraph.gz 28.9 Mb (ftp)(http) BEDGRAPH
GSE23260_emb-bwm-v2_tars_maxgap30_minrun100_thresh8.65.gff3.gz 1.0 Mb (ftp)(http) GFF3
Processed data are available on Series record

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