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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 06, 2023 |
| Title |
RNA-seq profiling of mammary epithelial cell populations from Brca2 knockout and wildtype mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Brca2(fl/fl)Trp53(fl/fl) mice were crossed with MMTV-cre mice to create mice with both alleles of Brca2 and one allele of Trp53 deleted (refered to as Brca2KO). Mature luminal, luminal progenitor and basal mammary epithelial cell populations were sorted from Brca2KO and wildtype mice. Transcriptional profiling revealed marked perturbation within the luminal Brca2KO compartment
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| Overall design |
Bulk RNA-sequencing was performed on FACS-isolated mature luminal, luminal progenitor and basal mammary epithelial cell populations from 14 week old MMTV-cre(T/+)Brca2(delta/delta)Trp53(delta/+) mice (n=3 individual mice) and wildtype controls (n=3 individual mice). All mice were female.
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| Contributor(s) |
Li M, Smyth GK, Joyce R, Pascual R, Lindeman GJ, Visvader JE |
| Citation(s) |
38216737 |
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| Submission date |
May 16, 2023 |
| Last update date |
Mar 06, 2024 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
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| Phone |
(+61 3) 9345 2326
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| Fax |
(+61 3) 9347 0852
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| URL |
http://www.wehi.edu.au
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| Organization name |
Walter and Eliza Hall Institute of Medical Research
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| Department |
Bioinformatics
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| Lab |
Smyth
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| Street address |
1G Royal Pde
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| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (18)
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| GSM7360210 |
Mammary epithelium basal cells, MMTVcre/Brca2/Trp53 knockdown, bio rep 1 |
| GSM7360211 |
Mammary epithelium luminal progenitors, MMTVcre/Brca2/Trp53 knockdown, bio rep 1 |
| GSM7360212 |
Mammary epithelium mature luminal cells, MMTVcre/Brca2/Trp53 knockdown, bio rep 1 |
| GSM7360213 |
Mammary epithelium basal cells, wildtype, bio rep 1 |
| GSM7360214 |
Mammary epithelium luminal progenitors, wildtype, bio rep 1 |
| GSM7360215 |
Mammary epithelium mature luminal cells, wildtype, bio rep 1 |
| GSM7360216 |
Mammary epithelium basal cells, MMTVcre/Brca2/Trp53 knockdown, bio rep 2 |
| GSM7360217 |
Mammary epithelium luminal progenitors, MMTVcre/Brca2/Trp53 knockdown, bio rep 2 |
| GSM7360218 |
Mammary epithelium mature luminal cells, MMTVcre/Brca2/Trp53 knockdown, bio rep 2 |
| GSM7360219 |
Mammary epithelium basal cells, wildtype, bio rep 2 |
| GSM7360220 |
Mammary epithelium luminal progenitors, wildtype, bio rep 2 |
| GSM7360221 |
Mammary epithelium mature luminal cells, wildtype, bio rep 2 |
| GSM7360222 |
Mammary epithelium basal cells, MMTVcre/Brca2/Trp53 knockdown, bio rep 3 |
| GSM7360223 |
Mammary epithelium luminal progenitors, MMTVcre/Brca2/Trp53 knockdown, bio rep 3 |
| GSM7360224 |
Mammary epithelium mature luminal cells, MMTVcre/Brca2/Trp53 knockdown, bio rep 3 |
| GSM7360225 |
Mammary epithelium basal cells, wildtype, bio rep 3 |
| GSM7360226 |
Mammary epithelium luminal progenitors, wildtype, bio rep 3 |
| GSM7360227 |
Mammary epithelium mature luminal cells, wildtype, bio rep 3 |
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| Relations |
| BioProject |
PRJNA973059 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE232631_GenewiseCounts.txt.gz |
687.0 Kb |
(ftp)(http) |
TXT |
| GSE232631_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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