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Series GSE23306 Query DataSets for GSE23306
Status Public on Aug 22, 2010
Title The JMJD3-IRF4 axis regulates M2 macrophage polarization and host responses against helminth infection
Organism Mus musculus
Experiment type Expression profiling by array
Genome binding/occupancy profiling by high throughput sequencing
Summary Polarization of macrophages to M1 or M2 cells is important for mounting responses against bacterial and helminth infection respectively. Jumonji domain containing 3 (JMJD3), a histone 3 K27 demethylase, has been implicated in the activation of macrophages. Here we show that JMJD3 is essential for M2 macrophage polarization to helminth infection and chitin, though JMJD3 is dispensable for M1 responses. Furthermore, Jmjd3 is critical for proper bone marrow macrophage differentiation in a demethylase activity-dependent manner. Jmjd3 deficiency affected trimethylation of H3K27 in only a limited numbers of genes. Among them, we identified Irf4 as the target transcription factor critical for controlling M2 macrophage polarization. Collectively, these results show that JMJD3-mediated H3K27 demethylation is critical for regulating M2 macrophage development leading to anti-helminth host responses.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
 
Citation(s) 20729857
Submission date Jul 29, 2010
Last update date May 15, 2019
Contact name Osamu Takeuchi
E-mail(s) otake@mfour.med.kyoto-u.ac.jp
Phone 81-75-753-9500
Organization name Kyoto University
Department Grad School of Medicine
Lab Medical Chemistry
Street address Yoshida-konoe-cho, Sakyo-ku,
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platforms (2)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL10010 AB SOLiD System (Mus musculus)
Samples (6)
GSM570692 Wild-type unstimulated
GSM570693 Wild-type LPS
GSM570694 Jmjd3-/- unstimulated
This SuperSeries is composed of the following SubSeries:
GSE23180 Expression data of LPS-stimulated bone marrow macrophages induced by M-CSF from wild-type and Jmjd3-/- mice.
GSE23297 Genome wide trimethyl-H3K27 distibution in macrophages from wild-type and Jmjd3-/- macrophages.
Relations
BioProject PRJNA131203

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23306_RAW.tar 225.8 Mb (http)(custom) TAR (of BED, CEL)
SRA Run SelectorHelp

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