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Series GSE23309 Query DataSets for GSE23309
Status Public on Jul 30, 2010
Title Sex-biased expression in heads of three Drosophila species
Organisms Drosophila melanogaster; Drosophila mojavensis; Drosophila pseudoobscura
Experiment type Expression profiling by array
Summary Investigation of sex-biased expression across species have relied on measurements from whole flies which sample the extensive expression differences found in the germline and gonads of females and males. We wanted to examine genes with sex-biased expression in a somatic tissue to analyze patterns of genes with sex-biased expression in the context of a tissue more phenotypically similar between females and males. We used a Nimblegen microarray designed for Drosophila melanogaster and two custom micoarrays designed for D. pseudoobscura and D. mojavensis to survey gene expression differences in heads of females and males.
 
Overall design PolyA+ mRNA was isolated in quadruplicate from dissected heads of 8 day old adult females and males. Female and male mRNA were labeled with Cy3 or Cy5 dyes separately and co-hybridized to species-specific microarrays. We analyzed a total of 24 channels of data.
 
Contributor(s) Malone JH, Oliver B
Citation(s) 21627854, 22499666
Submission date Jul 29, 2010
Last update date Sep 17, 2012
Contact name Brian Oliver
E-mail(s) briano@nih.gov
Phone 301-204-9463
Organization name NIDDK, NIH
Department LBG
Lab Developmental Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (3)
GPL4629 Nimblegen Drosophila melanogaster expression array/2005-10-17_Dmel4_60mer_exp
GPL4630 Nimblegen Dmoj expression custom array/2005-06-03_D_mojavensis_50mer
GPL4631 Nimblegen Dpse expression custom array/2005-06-03_D_pseudoobscura_50mer
Samples (24)
GSM571913 Dmel_F_R1
GSM571914 Dmel_F_R2
GSM571915 Dmel_F_R3
Relations
BioProject PRJNA131201

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23309_Dmel_GEO_RAW.txt.gz 10.8 Mb (ftp)(http) TXT
GSE23309_Dmoj_GEO_RAW.txt.gz 9.0 Mb (ftp)(http) TXT
GSE23309_Dpse_GEO_RAW.txt.gz 9.4 Mb (ftp)(http) TXT
GSE23309_RAW.tar 327.8 Mb (http)(custom) TAR (of FTR, PAIR)
Processed data included within Sample table

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