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Series GSE233464 Query DataSets for GSE233464
Status Public on Jun 17, 2024
Title The impact of conditional Opa1 Knockout in neural stem cells on transcriptome changes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the function of Opa1 in the regulation of adult neurogenesis, we created a conditional Opa1-knockout mouse line by cross-breeding Nestin-CreERT2;ROSA26YFP mice with Opa1-Flox mice.
Using Tamoxifen, we induced Opa1 knockout on 8-weeks-old Nestin-CreERT2;ROSA26YFP;Opa1-Flox mice. We then sorted YFP-positive cells from the hippocampus using flow cytometry at 10 weeks. RNA was extracted from sorted cells and subjected to next generation sequencing.
Overall design Comparative gene expression profiling analysis of RNA-seq data for WT and Opa1-KO neural stem cells.
Contributor(s) Slack R, Iqbal MA
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Submission date May 25, 2023
Last update date Jun 17, 2024
Contact name Ruth Slack
Phone 6135625800
Organization name University of Ottawa
Department Cellular and Molecular Medicine
Street address 451 Smyth Road
City Ottawa
State/province Ontario
ZIP/Postal code K1H 8M5
Country Canada
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM7428961 YFP-positive neural stem cells, WT1
GSM7428962 YFP-positive neural stem cells, WT2
GSM7428963 YFP-positive neural stem cells, Opa1KO1
This SubSeries is part of SuperSeries:
GSE233465 The impact of conditional Opa1 Knockout
BioProject PRJNA976313

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Supplementary file Size Download File type/resource
GSE233464_2023-05-11_Opa1KO_RNA-seq_2reps.xlsx 5.5 Mb (ftp)(http) XLSX
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