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Series GSE23367 Query DataSets for GSE23367
Status Public on Jan 01, 2013
Title A non-classical LysR type transcriptional regulator PA2206 is required for an effective oxidative stress response in Pseudomonas aeruginosa.
Organism Pseudomonas aeruginosa
Experiment type Expression profiling by array
Summary LysR-type transcriptional regulators (LTTRs) are emerging as key circuit components in regulating microbial stress responses and are implicated in modulating oxidative stress in the human opportunistic pathogen Pseudomonas aeruginosa. The oxidative stress response encapsulates several strategies to overcome the deleterious effects of reactive oxygen species. However, many of the regulatory components and associated molecular mechanisms underpinning this key adaptive response remain to be characterised. Comparative analysis of publically available transcriptomic datasets led to the identification of a novel LTTR, PA2206, whose expression was altered in response to a range of host signals in addition to oxidative stress. PA2206 was found to be required for tolerance to H2O2 in vitro and lethality in vivo in the Zebrafish embryo model of infection. Transcriptomic analysis in the presence of H2O2 showed that PA2206 altered the expression of 58 genes, including a large repertoire of oxidative stress and iron responsive genes, independent of the master regulator of oxidative stress, OxyR. Contrary to the classic mechanism of LysR regulation, PA2206 did not autoregulate its own expression and did not influence expression of adjacent or divergently transcribed genes. The PA2214-15 operon was identified as a direct target of PA2206 with truncated promoter fragments revealing binding to the 5’-ATTGCCTGGGGTTAT-3’ LysR box adjacent to the predicted -35 region. PA2206 also interacted with the pvdS promoter suggesting a global dimension to the PA2206 regulon, and suggests PA2206 is an important regulatory component of P. aeruginosa adaptation during oxidative stress.
 
Overall design Six samples were analysed in total, three biological replicates of the PA2206 mutant and PA2206 complemented strains. Each biological replicate included three technical replicates.
 
Contributor(s) Haynes JM, Reen FJ, Mooij MJ, O’Gara F
Citation(s) 23382903
Submission date Aug 02, 2010
Last update date Jul 06, 2016
Contact name Fergal O'Gara
E-mail(s) j.haynes@ucc.ie
Phone 00 353 21 4901330
Organization name BIOMERIT Research Centre
Department Microbiology
Street address University College Cork
City Cork
ZIP/Postal code IRE
Country Ireland
 
Platforms (1)
GPL84 [Pae_G1a] Affymetrix Pseudomonas aeruginosa Array
Samples (6)
GSM573322 PA2206 mutant_H2O2_10mins_rep1
GSM573323 PA2206 mutant_H2O2_10mins_rep2
GSM573324 PA2206 complemented strain_H2O2_10mins_rep1
Relations
BioProject PRJNA131241

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23367_RAW.tar 4.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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