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| Status |
Public on Jul 08, 2011 |
| Title |
Transcriptomes of monocyte-derived DCs stimulated with various compounds |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Little is known about the early transcriptional events in innate immune signaling in immature and tolerogenic monocyte-derived dendritic cells (DCs), the professional antigen-presenting cells of our immune system. TLR ligands usually induce a proinflammatory transcriptional response, whereas IL10 and/or dexamethasone induce a more tolerogenic phenotype.
We used Affymetrix microarrays to obtain detailed information underlying pro- and anti-inflammatory transcriptional responsesand transcriptional networks in DCs
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| Overall design |
A pilot experiment was performed in which monocyte-derived DCs were either treated with TLR4 ligand LPS, or IL10 and dexamethason. Furthermore, IL10/dexamethason treated cells were also stimulated with LPS for an additional 6 hr. All samples were then subjected to global gene expression analysis using Affymetrix technology.
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| Contributor(s) |
Jansen BJ, Eleveld-Trancikova D, van Hout-Kuijer M, Adema GJ, Sama I, Martijn H |
| Citation(s) |
21708028 |
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| Submission date |
Aug 02, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Bastiaan Jansen |
| E-mail(s) |
bjhjansen@gmail.com
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| Organization name |
Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Center Nijmegen
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| Department |
Tumor Immunology
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| Street address |
PO Box 9101
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| City |
Nijmegen |
| ZIP/Postal code |
6500 HB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| GSM573357 |
Immature DCs, technical replicate 1 |
| GSM573358 |
Immature DCs, technical replicate 2 |
| GSM573359 |
Immature DCs, technical replicate 3 |
| GSM573360 |
Maturing DCs, technical replicate 1 |
| GSM573361 |
Maturing DCs, technical replicate 2 |
| GSM573362 |
Maturing DCs, technical replicate 3 |
| GSM573363 |
Tolerogenic DCs, technical replicate 1 |
| GSM573364 |
Tolerogenic DCs, technical replicate 2 |
| GSM573365 |
Tolerogenic DCs, technical replicate 3 |
| GSM573366 |
Activated tolerogenic DCs, technical replicate 1 |
| GSM573367 |
Activated tolerogenic DCs, technical replicate 2 |
| GSM573368 |
Activated tolerogenic DCs, technical replicate 3 |
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| Relations |
| BioProject |
PRJNA131287 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23371_RAW.tar |
44.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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